[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample
2DNOESY with N15 C13 Labelled sample or Unlabelled sample
Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ?
Thanks & Regards Srinivas
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nmrlearner
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02-06-2012 03:54 PM
Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks
Abstract 1H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t = 0â??4 h) between blood collection and processing and of the time from processing to freezing (up to 24 h). The stability of the urine...
[Question from NMRWiki Q&A forum] What is the optimal sample length with Shigemi tubes?
What is the optimal sample length with Shigemi tubes?
Our coil area length is 16 mm. Should we be making samples exactly 16 mm long in the Shigemi tubes for the best sensitivity (assuming that we are limited on amount of material)?
For example, we use D2O matched shigemi tubes for samples in 90/10 H2O/D2O. Is susceptibility matching affected by switch from D2O to H2O?
Thanks!
nmrlearner
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08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] How much benefit to expect from best NMR tubes?
How much benefit to expect from best NMR tubes?
Hello, how much benefit in resolution and signal-to-noise will we get when using 800 MHz rated tubes (on the 800 MHz instrument) vs 500+ MHz rated tubes?
Is it worth spending the extra money on ultra-high quality tubes for routine work at 800 MHz?
Thanks.
nmrlearner
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08-22-2010 02:30 AM
[Stan NMR blog] NMR tubes, or how to get poor very fast
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