Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methylâ??methyl nuclear overhauser enhancement spectroscopy
Automated sequence- and stereo-specific assignment of methyl-labeled proteins by paramagnetic relaxation and methylâ??methyl nuclear overhauser enhancement spectroscopy
Abstract Methyl-transverse relaxation optimized spectroscopy is rapidly becoming the preferred NMR technique for probing structure and dynamics of very large proteins up to ~1 MDa in molecular size. Data interpretation, however, necessitates assignment of methyl groups which still presents a very challenging and time-consuming process. Here we demonstrate that, in combination with a known 3D structure, paramagnetic...
[Question from NMRWiki Q&A forum] RDCs di-Methyl lysine
RDCs di-Methyl lysine
We are interested in studying di-methyl lysine, given that in most cases the two methyl groups are equivalent and each have the three protons, how much and if so what, information could you realistically get from RDCs on the Di- methyl signals?
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03-30-2011 09:11 PM
[NMR paper] Amino acid-type edited NMR experiments for methyl-methyl distance measurement in 13C-
Amino acid-type edited NMR experiments for methyl-methyl distance measurement in 13C-labeled proteins.
Related Articles Amino acid-type edited NMR experiments for methyl-methyl distance measurement in 13C-labeled proteins.
J Am Chem Soc. 2004 Aug 11;126(31):9584-91
Authors: Van Melckebeke H, Simorre JP, Brutscher B
New NMR experiments are presented for the measurement of methyl-methyl distances in (13)C-labeled proteins from a series of amino acid-type separated 2D or 3D NOESY spectra. Hadamard amino acid-type encoding of the proximal methyl...
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11-24-2010 10:01 PM
[NMR paper] NMR studies of the methionine methyl groups in calmodulin.
NMR studies of the methionine methyl groups in calmodulin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR studies of the methionine methyl groups in calmodulin.
FEBS Lett. 1995 Jun 12;366(2-3):104-8
Authors: Siivari K, Zhang M, Palmer AG, Vogel HJ
Calmodulin (CaM) is a ubiquitous Ca(2+)-binding protein that can regulate a wide variety of cellular events. The protein contains 9 Met out of a total of 148 amino acid residues. The binding of Ca2+ to CaM induces...
Do the three classes of methyl S2 really exist?
The Origin of Protein Sidechain Order Parameter Distributions
Robert B. Best, Jane Clarke, and Martin Karplus
J. Am. Chem. Soc.; 2004; 126(25) pp 7734 - 7735
http://pubs.acs.org/isubscribe/journals/jacsat/126/i25/figures/ja049078wn00001.gif
Abstract:
Previous work by Wand et al. (Nature 2001, 411, 501-504) showed that the NMR order parameters characterizing the amplitude of motion of protein side chains seemed to form a multimodal distribution. At the time, no detailed explanation of this at the molecular level was offered, yet three "classes" of motion were inferred. We have...
Quiz 1 - NMR Methyl methanoate
Linear Mode
