An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media
An efficient protocol for incorporation of an unnatural amino acid in perdeuterated recombinant proteins using glucose-based media
Abstract The in vivo incorporation of unnatural amino acids into proteins is a well-established technique requiring an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is incorporated at a position encoded by a TAG amber codon. Although this technology provides unique opportunities to engineer protein structures, poor protein yields are usually obtained in deuterated media, hampering its application in the protein NMR...
nmrlearner
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02-21-2012 03:40 AM
MRI of mass transport in porous media: drying and sorption processes
MRI of mass transport in porous media: drying and sorption processes
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 14 December 2011</br>
Igor V.*Koptyug</br>
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nmrlearner
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12-16-2011 01:23 PM
Domain cooperativity in multidomain proteins: what can we learn from molecular alignment in anisotropic media?
Domain cooperativity in multidomain proteins: what can we learn from molecular alignment in anisotropic media?
Abstract Many proteins have modular design with multiple globular domains connected via flexible linkers. As a simple model of such system, we study a tandem construct consisting of two identical SH3 domains and a variable-length Gly/Ser linker. When the linker is short, this construct represents a dumbbell-shaped molecule with limited amount of domainā??domain mobility. Due to its elongated shape, this molecule efficiently aligns in steric alignment media. As the length of...
Nuclear Spin Relaxation in Isotropic and Anisotropic Media
Nuclear Spin Relaxation in Isotropic and Anisotropic Media
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 29 April 2010</br>
Matthew P., Nicholas , Ertan, Eryilmaz , Fabien, Ferrage , David, Cowburn , Ranajeet, Ghose</br>
We present a unified framework to describe nuclear spin relaxation in isotropic and anisotropic solutions for single-domain diffusers. All expressions, diffusion and net spin-relaxation theory are derived ab initio. Complete analytical expressions are provided wherever...
nmrlearner
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08-16-2010 03:50 AM
De novo determination of internuclear vector orientations from residual dipolar couplings measured in three independent alignment media
De novo determination of internuclear vector orientations from residual dipolar couplings measured in three independent alignment media
Ke Ruan, Kathryn B. Briggman and Joel R. Tolman
Journal of Biomolecular NMR; 2008; 41(2) pp 61 - 76
Abstract:
The straightforward interpretation of solution state residual dipolar couplings (RDCs) in terms of internuclear vector orientations generally requires prior knowledge of the alignment tensor, which in turn is normally estimated using a structural model. We have developed a protocol which allows the requirement for prior structural knowledge to...
daniel
Journal club
0
08-03-2008 03:54 AM
Composite Alignment Media for the Measurement of Independent Sets of NMR Residual Dipolar Couplings
Composite Alignment Media for the Measurement of Independent Sets of NMR Residual Dipolar Couplings
Ke Ruan and Joel R. Tolman
J. Am. Chem. Soc.; 2005; 127(43) pp 15032 - 15033;
Abstract:
The measurement of independent sets of NMR residual dipolar couplings (RDCs) in multiple alignment media can provide a detailed view of biomolecular structure and dynamics, yet remains experimentally challenging. It is demonstrated here that independent sets of RDCs can be measured for ubiquitin using just a single alignment medium composed of aligned bacteriophage Pf1 particles embedded in a...
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