In the p3919gp the number of scans is a multiple of 8 which makes optimizing this experiment a bit of a pain. can anyone explain why ns needs to be a multiple of 8
thx
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[NMR paper] Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Multiple Scale Dynamics in Proteins Probed at Multiple Time Scales through Fluctuations of NMR Chemical Shifts.
J Phys Chem B. 2014 Mar 14;
Authors: Calligari PA, Abergel D
Abstract
Fluctuations of NMR resonance frequency shifts and their relation with protein exchanging conformations are usually...
[Louic Vermeer blog] Using procheck_nmr on a large number of structures
Using procheck_nmr on a large number of structures
After a structure calculation with the aria 2.2 software I used aqua and procheck_nmr to assess the result. Although procheck_nmr worked fine on my 20 refined structures (in aria’s refine directory), it ran into trouble with the 100 structures after iteration 8 (directory it8): * Restraints read in from file: * allpdb.nrv
Read complete story on Louic Vermeer blog
nmrlearner
News from NMR blogs
0
10-18-2010 09:17 AM
[U. of Ottawa NMR Facility Blog] Dummy Scans
Dummy Scans
Dummy scans (DS, Bruker) or steady state scans (SS, Varian) are scans taken in an NMR acquisition before the receiver is turned on and data are collected. Each dummy scan contains all of the rf pulses, delays and gradients used in the pulse program; the only difference is that the receiver is not turned on to collect data. Dummy scans are typically used to ensure that a spin system is in a steady state before data are collected. For example, One may collect a spectrum using a relaxation delay short with respect to the T1's of some of the resonances in the spectrum. The first...
nmrlearner
News from NMR blogs
0
08-21-2010 08:15 PM
[U. of Ottawa NMR Facility Blog] Watergate vs Presaturation
Watergate vs Presaturation
Biochemists and protein chemists are often interested in observing the NH protons in their samples. Since the NH protons usually undergo slow chemical exchange with water, it is desirable to run the samples in H2O rather than D2O so the NH protons will not exchange with the deuterium in the solvent which would make them invisible in the 1H NMR spectrum. In practice, a mixture of 10% D2O and 90% H2O is used as a solvent so that a deuterium lock can be established and used while running the spectrum. The very high concentration of water compared to the very low...
nmrlearner
News from NMR blogs
0
08-21-2010 08:15 PM
[U. of Ottawa NMR Facility Blog] Watergate
WATERGATE
WATER suppression by GrAdient Tailored Excitation (WATERGATE) is a clever technique used to suppress the water signal in an aqueous sample. It is widely used in many complicated pulse sequences. Unlike presaturation which irradiates the water resonance with a long low power pulse, this method is based on the gradient spin echo technique used also to measure diffusion constants and DOSY spectra. The pulse sequence is shown here:http://1.bp.blogspot.com/_5wBTR2kKTqA/S3GsqqD3wiI/AAAAAAAAAuM/gXnd8Ss-KPg/s400/watergate1_1.jpgThe transmitter frequency is set on the water resonance. A...
nmrlearner
News from NMR blogs
0
08-21-2010 08:15 PM
peak intensity vs number of scans
Hi,
I have a question regarding how the peak intensities change when with increase in number of scans when the data is collected on a Varian NMR spectrometer. Suppose I collect a 15N-1H HSQC spectra with 8 scans and then collect the same spectra with 16 scans, would the peak intensities be double in the 16 scan spectra as compared to the 8 scan spectra? Does anyone know how the varian spectrometer scales the intensities according to the number of scans? Any suggestion or reply to my query will be extremely useful and greatly appreciated.
Thanks.
aish1982
NMR software
0
08-08-2008 11:56 PM
peak intensity vs number of scans
Hi,
I have a question regarding how the peak intensities change when with increase in number of scans when the data is collected on a Varian NMR spectrometer. Suppose I collect a 15N-1H HSQC spectra with 8 scans and then collect the same spectra with 16 scans, would the peak intensities be double in the 16 scan spectra as compared to the 8 scan spectra? Does anyone know how the varian spectrometer scales the intensities according to the number of scans? Any suggestion or reply to my query will be extremely useful and greatly appreciated.
Thanks.
why is the number of scans in watergate a multiple of 8?
Linear Mode
