Question from NMRWiki Q&A forum Why is HNCO the most sensitive Spectrum?

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[Question from NMRWiki Q&A forum] Why is HNCO the most sensitive Spectrum?

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Structure from NMR restraints:

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Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

Refinement:

Amber

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Torsion angles from chemical shifts:

Preditor

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Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

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HADDOCK

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V-NMR

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Methyl S2

B-factor

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Antechamber

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Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

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Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

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CCPN

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Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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04-17-2013, 08:15 PM

nmrlearner

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Why is HNCO the most sensitive Spectrum?

Why is HNCO the most sensitive Spectrum?

I would like to know where the advantages of the HNCO spectrum stem from. I read that it is the most sensitive multi-D NMR spectrum, but all i know is, that it produces an absorptive spectrum, which is an obvious advantage.

Can someone explain to me why it is the most sensitive 3D-NMR spectrum and why it is popular?

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