Question from NMRWiki Q&A forum Why does Lift not work?

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] Why does Lift not work?

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-02-2015, 01:05 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Why does Lift not work?

Why does Lift not work?

Dear all,we have newly installed Bruker Ascend 600 with Sample Jet. Last month, we had a compressor error and NMR was out of order. This week I try to revive our spectrometer, it seems everything ok except LIFT. after 'ej' I do not hear any sound indicating any change in gas flow, the same after 'ij' When changing gas flow in VTU from e.g. 300lph to 500 lph, system reacts and the change is visible in gas flow monitoring and also noticeble with fingers over hole. However, after 'ej' I see only minor changes in gas flow..Do you have any idea what to do? I will be very thankfull

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] Avance III 400 NMR lift unresponsive... emergency air lift activiated Avance III 400 NMR lift unresponsive... emergency air lift activiated Hi, I am unable to activate our lift on our avance III 400 NMR from BruKer The message is emergency air lift activated. Both the inject and eject commands give this error. And I am unable to hear of air flow through the lift or any other mechanism when a lift is attempted. We could still lock on and shim to the chloroform peak. I've gone through a couple of trouble shooting pathways but so far I'm unsuccessful and getting our lift to work and shutting off the emergency air flow. Does any one have any suggestions? ...	nmrlearner	News from other NMR forums	0	05-25-2013 12:05 PM
[NMR images] An NMR spectrometer can work http://www.avogadro.co.uk/analysis/nmr/nmr.gif avogadro.co.uk 6/07/2011 6:15:13 AM GMT An NMR spectrometer can work More...	nmrlearner	NMR pictures	0	07-30-2011 11:50 AM
[NMR photos] Take your son or daughter to work MRI http://farm6.static.flickr.com/5027/5667828189_3cd52920f9.jpg Take your son or daughter to work MRI More...	nmrlearner	NMR pictures	0	04-29-2011 04:31 PM
Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins. Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins. Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins. J Am Chem Soc. 2011 Feb 2; Authors: Shi L, Traaseth NJ, Verardi R, Gustavsson M, Gao J, Veglia G Residual dipolar couplings (RDCs) are widely used as orientation-dependent NMR restraints to improve the resolution of the NMR conformational ensemble of biomacromolecules and define the...	nmrlearner	Journal club	0	02-04-2011 11:34 AM
Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins Lei Shi, Nathaniel J. Traaseth, Raffaello Verardi, Martin Gustavsson, Jiali Gao and Gianluigi Veglia http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja109080t/aop/images/medium/ja-2010-09080t_0003.gif Journal of the American Chemical Society DOI: 10.1021/ja109080t http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/Tu9H79dfKCk	nmrlearner	Journal club	0	02-03-2011 06:45 AM
[NMR software blog] Men at Work Men at Work As soon as I began experimenting with the DOSY toolbox I was surprised to discover an unexpected hole, which is just a novel proof of my old theory on NMR imprinting. If your first spectrometer is a Varian, you will become a certain kind of spectroscopist and a certain kind of programmer. If your first instrument is a Bruker, you will become a different spectroscopist and a different programmer. If a brukerist programmer had written this toolbox, he would have imported the frequency domain spectrum and started from there. Mathias Nilsson is instead a Varianist and he imports...	nmrlearner	News from NMR blogs	0	08-21-2010 06:29 PM
[Stan NMR blog] EPR at Work EPR at Work Pointer to an important source of historic and educational EPR/ESR documantation More...	nmrlearner	News from NMR blogs	0	08-21-2010 05:42 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off