I'm trying to modify some pulse sequence on bruker 400, and is confused with the use of 'blkgrad' and 'unblkgrad'; and ,what is the difference between 'blkgrad' and 'groff'?
Check if somebody has answered this question on NMRWiki QA forum
[Question from NMRWiki Q&A forum] How to performing acquisition during all time multi pulse sequence - Bruker ?
How to performing acquisition during all time multi pulse sequence - Bruker ?
Hi all, I have a small question on how to aquired a complete signal in BRUKER AVIII.I want to create a multi pulse sequence (1D), where I want to aquire the signal (sequentially) do not adding or replacing the data before each pulse.
||__________||_________||__________||___________ ...
tp tp tp tp ...Performing Aquisition all time.................
|| - pulse
nmrlearner
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10-10-2013 09:27 AM
[NMRpipe Yahoo group] Looking for help on modifying pulse sequence to enable non-uniformly
Looking for help on modifying pulse sequence to enable non-uniformly
Hello everyone, I would like to try recording MDs by using non-uniformly sampling on Varian VNMRJ 600(SOLID),Is there anyone have some experiences of modifying
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03-12-2012 03:05 PM
[Question from NMRWiki Q&A forum] CT COSY pulse sequence programming
CT COSY pulse sequence programming
Hi,
I want to make a CT-COSY pulse sequence. But I'm bother by incremented delay. When I type in the BaseLevelRelation.h this:
IN=-INand add in the microprogram the delay before and after my pi pulse,it didn't work... It seems like it calculate the d28 delay from the IN28 increment, but doesn't want to apply this delay in the pulse sequence....Any idea ? Perhaps it's about some incrementation declaration or something like that ?
nmrlearner
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05-04-2011 05:33 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues!
Micropost: Pulse Sequence Blues!
Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity:
Pulse Sequence Blues!
NMR can also be fun :-)
https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com
nmrlearner
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02-23-2011 11:21 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues!
Micropost: Pulse Sequence Blues!
Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity:
Pulse Sequence Blues!
NMR can also be fun :-)
https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl
Best pulse sequence for HSQC in Guanidine-HCl
Hello,
I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63.
Using WGgNhsqc, the protein...