Question from NMRWiki Q&A forum When to use 'BLKGRAD' and 'UNBLKGRAD' in a bruker pulse sequence ?

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] When to use 'BLKGRAD' and 'UNBLKGRAD' in a bruker pulse sequence ?

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-25-2016, 04:12 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

When to use 'BLKGRAD' and 'UNBLKGRAD' in a bruker pulse sequence ?

When to use 'BLKGRAD' and 'UNBLKGRAD' in a bruker pulse sequence ?

I'm trying to modify some pulse sequence on bruker 400, and is confused with the use of 'blkgrad' and 'unblkgrad'; and ,what is the difference between 'blkgrad' and 'groff'?

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR images] Cosy Nmr Pulse Sequence - Website of jadatide! http://www.chemistry.nmsu.edu/instrumentation/nmr_cosy3.gif jadatide.jimdo.com 17/10/2013 4:58:43 PM GMT Cosy Nmr Pulse Sequence - Website of jadatide! More...	nmrlearner	NMR pictures	0	10-17-2013 04:57 PM
[Question from NMRWiki Q&A forum] How to performing acquisition during all time multi pulse sequence - Bruker ? How to performing acquisition during all time multi pulse sequence - Bruker ? Hi all, I have a small question on how to aquired a complete signal in BRUKER AVIII.I want to create a multi pulse sequence (1D), where I want to aquire the signal (sequentially) do not adding or replacing the data before each pulse. \|\|__________\|\|_________\|\|__________\|\|___________ ... tp tp tp tp ...Performing Aquisition all time................. \|\| - pulse	nmrlearner	News from other NMR forums	0	10-10-2013 09:27 AM
[NMRpipe Yahoo group] Looking for help on modifying pulse sequence to enable non-uniformly Looking for help on modifying pulse sequence to enable non-uniformly Hello everyone, I would like to try recording MDs by using non-uniformly sampling on Varian VNMRJ 600(SOLID),Is there anyone have some experiences of modifying More...	NMRpipe Yahoo group news	News from other NMR forums	0	03-12-2012 03:05 PM
[Question from NMRWiki Q&A forum] CT COSY pulse sequence programming CT COSY pulse sequence programming Hi, I want to make a CT-COSY pulse sequence. But I'm bother by incremented delay. When I type in the BaseLevelRelation.h this: IN=-INand add in the microprogram the delay before and after my pi pulse,it didn't work... It seems like it calculate the d28 delay from the IN28 increment, but doesn't want to apply this delay in the pulse sequence....Any idea ? Perhaps it's about some incrementation declaration or something like that ?	nmrlearner	News from other NMR forums	0	05-04-2011 05:33 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues! Micropost: Pulse Sequence Blues! Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity: Pulse Sequence Blues! NMR can also be fun :-) https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com	nmrlearner	News from NMR blogs	0	02-23-2011 11:21 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues! Micropost: Pulse Sequence Blues! Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity: Pulse Sequence Blues! NMR can also be fun :-) https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com	nmrlearner	News from NMR blogs	0	02-23-2011 11:18 PM
[NMRwiki tweet] nmrwiki: How to set spinlock power for the @bruker roesyph.2 #nmr pulse sequence?http://qa.nmrwiki.org/question/219/ nmrwiki: How to set spinlock power for the @bruker roesyph.2 #nmr pulse sequence?http://qa.nmrwiki.org/question/219/ nmrwiki: How to set spinlock power for the @bruker roesyph.2 #nmr pulse sequence?http://qa.nmrwiki.org/question/219/ Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	01-18-2011 02:44 AM
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl Best pulse sequence for HSQC in Guanidine-HCl Hello, I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63. Using WGgNhsqc, the protein...	nmrlearner	News from other NMR forums	0	10-23-2010 07:42 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off