Hi., i have one compound i need only two bond correlation contours from quaternary carbon to proton. Is there any pulse sequence to find only two bond correlations ie, from quaternary carbon to protons with minimum amount of sample in "varian nmr".
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
Cross-Correlations Between Low-? Nuclei in Solids Via a Common Dipolar Bath
Cross-Correlations Between Low-? Nuclei in Solids Via a Common Dipolar Bath
Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 20 July 2011</br>
Aanatoly K., Khitrin , Jiadi, Xu , Ayyalusamy, Ramamoorthy</br>
Correlation of chemical shifts of low-? nuclei (such as 15N) is an important method for assignment of resonances in uniformly-labeled biological solids. Under static experimental conditions, an efficient mixing of low-? nuclear spin magnetization can be achieved by a thermal contact to the common reservoir of...
nmrlearner
Journal club
0
07-21-2011 10:31 PM
Improved accuracy in measuring one-bond and two-bond 15N,13Cα coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy
Improved accuracy in measuring one-bond and two-bond 15N,13Cα coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy
Abstract An extension to HN(CO-α/β-N,Cα-J)-TROSY (Permi and Annila in J Biomol NMR 16:221â??227, 2000) is proposed that permits the simultaneous determination of the four coupling constants 1 J Nâ?²(i)Cα(i), 2 J HN(i)Cα(i), 2 J Cα(iâ??1)Nâ?²(i), and 3 J Cα(iâ??1)HN(i) in 15N,13C-labeled proteins. Contrasting the original scheme, in which two separate subspectra exhibit the 2 J CαNâ?² coupling as inphase and antiphase splitting (IPAP), we...
nmrlearner
Journal club
0
06-10-2011 01:41 AM
[Question from NMRWiki Q&A forum] Correlations across glycosidic bonds
Correlations across glycosidic bonds
I am studying carbohydrates and was wanting to know if it was possible to record correlations through the ether linkage of the glycosidic bond in sugars either from the proton or carbon of one sugar top the adjacent sugar or the protons of a Serine or threonine side chain in a glyco-peptide. Would something like a 13C-HSQC-TOCSY cross the glycosidic bond? if not are there experiments that would?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
News from other NMR forums
0
03-19-2011 07:01 AM
[NMR paper] 1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C tim
1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
Related Articles 1H(C) and 1H(N) total NOE correlations in a single 3D NMR experiment. 15N and 13C time-sharing in t1 and t2 dimensions for simultaneous data acquisition.
J Biomol NMR. 2003 Nov;27(3):193-203
Authors: Xia Y, Yee A, Arrowsmith CH, Gao X
Simultaneous data acquisition in time-sharing (TS) multi-dimensional NMR experiments has been shown an effective means to reduce experimental...
nmrlearner
Journal club
0
11-24-2010 09:16 PM
[NMR paper] NMR studies on the structure/function correlations of T-cell-epitope analogs from per
NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin.
Related Articles NMR studies on the structure/function correlations of T-cell-epitope analogs from pertussis toxin.
Eur J Biochem. 1998 Jun 1;254(2):313-7
Authors: Scarselli M, Esposito G, De Magistris MT, Domenighini M, Rappuoli R, Burroni G, Bernini A, Niccolai N
A synthetic tridecapeptide, corresponding to the 30-42 fragment of the S1 subunit of pertussis toxin, has been structurally characterised by using NMR spectroscopy. The molecule...
nmrlearner
Journal club
0
11-17-2010 11:06 PM
HNCB Triple Resonance Experiment (Varian)
Hello,
Has anyone come across this experiment? I see it listed but have not been able to find the pulse sequence. I have noticed that in some cases that the reference simply implies the HNCACB experiment running in such a way to allow complete transfer to the CB.
So is there an HNCB or is the only HNCB in the form of the HNCACB with the delay set to allow complete transfer to the CB?
Thanks in advance!!!
element771
NMR Questions and Answers
0
07-22-2009 01:28 AM
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Simultaneous detection of amide and methyl correlations using a time shared NMR experiment: application to binding epitope mapping
Peter Würtz, Olli Aitio, Maarit Hellman and Perttu Permi
Journal of Biomolecular NMR; 2007; 39(2) pp 97 - 105
Abstract:
Simultaneous recording of different NMR parameters is an efficient way to reduce the overall experimental time and speed up structural studies of biological macromolecules. This can especially be beneficial in the case of fast NMR-based drug screening applications or for collecting NOE restraints, where prohibitively long data collection...