What is best pulse sequence in bio pack on vnmrj for C-13 edit NOESY ? I am working with 12 kd size protein which can exist as homo dimers ( i.e total size of homo-dimer is 24 kd ). Most frequent problems we are encountering as follows !line broadening , baseline , phase correction problems ( huge signal at 2.8 ppm )in first increments and f2f3 planes huge strips errors !
Thanking you in advance !
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Fig-1 1D projections fig-2 f1f3 projection in process in t1fig-3 f1f3 projection in process in t1 after transformation (1026-512-256)
<img width=50% src="http://qa.nmrwiki.org/upfiles/13204074491921939.png" /img>
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what is best pulse sequence for c13 edit noesy on vnmrj ?
Linear Mode
