Question from NMRWiki Q&A forum unresolved signals

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] unresolved signals

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-22-2016, 06:30 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

unresolved signals

unresolved signals

Hi allWe know that H NMR of paramagnetic sample or complex, is very broad with a bad signal to noise ratio. Do you have suggestion for obtain a better spectrum in case of paramagnetic sample ?Thank you

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[U. of Ottawa NMR Facility Blog] NMR Signals in Tuning Curves NMR Signals in Tuning Curves The quality of the tuning and matching of an NMR probe on a Bruker NMR spectrometer can be monitored by using the wobble (or "wobb") routine in the TOPSPIN software. This routine sweeps the frequency using low power and displays a plot corresponding roughly to the absorbance vs frequency for the probe electronics. If the probe has very high sensitivity (eg: a cryoprobe) and contains a sample rich in protons (eg: water) then one is able to observe the proton spectrum in the frequency swept wobble curve. This is demonstrated in the figure below which shows...	nmrlearner	News from NMR blogs	0	11-18-2015 03:25 PM
Extending the eNOE data set of large proteins by evaluation of NOEs with unresolved diagonals Extending the eNOE data set of large proteins by evaluation of NOEs with unresolved diagonals Abstract The representation of a proteinâ??s spatial sampling at atomic resolution is fundamental for understanding its function. NMR has been established as the best-suited technique toward this goal for small proteins. However, the accessible information content rapidly deteriorates with increasing protein size. We have recently demonstrated that for small proteins distance restraints with an accuracy smaller than 0.1Â*Ã? can be obtained by replacing...	nmrlearner	Journal club	0	03-08-2015 01:07 AM
[Question from NMRWiki Q&A forum] Weak NOE signals, serious t1 noise Weak NOE signals, serious t1 noise Since my university does not have a professional spectroscopist, I am a new learner of NMR, and I really have no one else to ask... I have a very concentrated sample (small molecule, MW < 300, 100 mg / mL), and I am running a 2D NOESY experiment (pulseprogram = noesyph in Bruker 400 MHz). However, the NOE signals that I am looking for are very weak, hidden by serious t1 noise (since the signal concerned is a CH3 group, very strong proton signal). Besides tricks like symmetrization, what else can I do to reduce the t1 noise or strengthen my NOE signal?...	nmrlearner	News from other NMR forums	0	10-26-2014 12:20 AM
[NMR images] The polarization value of enhanced signals, i.e. after ... http://tau.ep1.ruhr-uni-bochum.de/poltarg/target/nmr_setup.gif tau.ep1.ruhr-uni-bochum.de 18/06/2013 7:23:35 AM GMT The polarization value of enhanced signals, i.e. after ... More...	nmrlearner	NMR pictures	0	06-18-2013 07:21 AM
[NMR images] NMR The received signals are http://nationalnursingreview.com/wp-content/uploads/2009/11/NMR-224x300.jpg http://nationalnursingreview.com/category/instrumental-tests/ 25/03/2012 6:02:47 AM GMT NMR The received signals are More...	nmrlearner	NMR pictures	0	03-25-2012 06:02 AM
[NMR images] NMR The received signals are http://nationalnursingreview.com/wp-content/uploads/2009/11/NMR-224x300.jpg nationalnursingreview.com 30/10/2011 1:40:36 PM GMT NMR The received signals are More...	nmrlearner	NMR pictures	0	10-30-2011 01:39 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off