Question from NMRWiki Q&A forum Topspin 3.2.1 - 1D TOCSY in with differing mixing times

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] Topspin 3.2.1 - 1D TOCSY in with differing mixing times

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-03-2014, 10:46 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Topspin 3.2.1 - 1D TOCSY in with differing mixing times

Topspin 3.2.1 - 1D TOCSY in with differing mixing times

Hello,

I work a lot with compounds containing numerous carbohydrate groups. Therefore I use frequently 1D-TOCSY experiment with varying mixing times (parameter d9 = 30, 60 and 120 ms for selmlgp pulse program). It would be very useful to have a pseudo-2D experiment with these 3 1D-TOCSY spectra saved as one ser file (I remember that it was possible on Varian instruments).We've got a Bruker 500 MHz apparatus with Avance III HD console. Topspin version 3.2 pl1.Can you help me with creating such experiment? Is it even possible?Regards, Luke

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Professor Iain Campbell - The Times (subscription) http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcSAdL9ZRoebbX8jDDeV3ojUYVRkJA86Yy8FebJd-x5srFgc4OkebaaoOl4enfUUlxNi8Cl8LHRS The Times (subscription) <img alt="" height="1" width="1" /> Professor Iain Campbell The Times (subscription) The biophysicist Iain Campbell developed technologies which paved the way for new cancer drugs to be created. He was one of the first to use Nuclear Magnetic Resonance (NMR) technologies for studying the structure of proteins. Through this he ... Professor Iain Campbell - The Times (subscription)	nmrlearner	Online News	0	05-14-2014 04:50 AM
Why Are Lobsters Blue, and Why Does Cooking Turn Them Red? - New York Times <img alt="" height="1" width="1" /> Why Are Lobsters Blue, and Why Does Cooking Turn Them Red? New York Times ... the wavelengths of light that are absorbed. Using nuclear magnetic resonance imaging and spectroscopy to examine protein-pigment interactions, the researchers found that this effect was a large one, suggesting that it is the primary source of ... Why Are Lobsters Blue, and Why Does Cooking Turn Them Red? - New York Times More...	nmrlearner	Online News	0	09-17-2013 08:35 AM
[Question from NMRWiki Q&A forum] optimization of mixing time 3D NOESY experments optimization of mixing time 3D NOESY experments Could you please explain that how to optimize mixing time for 3D NOESY experiments for protein like C13 edit NOESY and N15 EDIT NOESY and i want keep spin diffusion as much as less ? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	09-21-2011 05:25 AM
[NMR paper] Effective rotational correlation times of proteins from NMR relaxation interference. Effective rotational correlation times of proteins from NMR relaxation interference. Related Articles Effective rotational correlation times of proteins from NMR relaxation interference. J Magn Reson. 2006 Jan;178(1):72-6 Authors: Lee D, Hilty C, Wider G, Wüthrich K Knowledge of the effective rotational correlation times, tauc, for the modulation of anisotropic spin-spin interactions in macromolecules subject to Brownian motion in solution is of key interest for the practice of NMR spectroscopy in structural biology. The value of tauc enables...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Mixing apparatus for preparing NMR samples under pressure. Mixing apparatus for preparing NMR samples under pressure. Related Articles Mixing apparatus for preparing NMR samples under pressure. J Magn Reson. 2003 Sep;164(1):84-91 Authors: Wu WJ, Vidugiris G, Mooberry ES, Westler WM, Markley JL The size limit for protein NMR spectroscopy in solution arises in large part from line broadening caused by slow molecular tumbling. One way to alleviate this problem is to increase the effective tumbling rate by reducing the viscosity of the solvent. Because proteins generally require an aqueous environment to...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
Homonuclear Mixing Sequences for Perdeuterated Proteins Homonuclear Mixing Sequences for Perdeuterated Proteins Publication year: 2010 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 26 October 2010</br> Kuo-Ying, Huang , Ansgar B., Siemer , Ann E., McDermott</br> We test the performance of several 13C homonuclear mixing sequences on perdeuterated microcrystalline ubiquitin. All sequences were applied without 1H decoupling and at relatively low MAS frequencies. We found that RFDR gave the highest overall transfer efficiency and that DREAM performs surprisingly well under these conditions being twice...	nmrlearner	Journal club	0	10-27-2010 08:51 AM
Broadband homonuclear TOCSY with amplitude and phase-modulated RF mixing schemes Broadband homonuclear TOCSY with amplitude and phase-modulated RF mixing schemes Anika Kirschstein, Christian Herbst, Kerstin Riedel, Michela Carella, Jörg Leppert, Oliver Ohlenschläger, Matthias Görlach and Ramadurai Ramachandran Journal of Biomolecular NMR; 2008; 40(4); pp 227-237 Abstract: We have explored the design of broadband scalar coupling mediated 13C–13C and cross-relaxation suppressed 1H–1H TOCSY sequences employing phase/amplitude modulated inversion pulses. Considering a variety of supercycles, pulsewidths and a RF field strength of 10 kHz, the Fourier coefficients...	Mikey	Journal club	0	08-14-2008 12:42 AM
HIFI-NMR: 10 times faster than regular NMR High-Resolution Iterative Frequency Identification for NMR as a General Strategy for Multidimensional Data Collection Hamid R. Eghbalnia, Arash Bahrami, Marco Tonelli, Klaas Hallenga, and John L. Markley J. Am. Chem. Soc.; 2005; 127(36) pp 12528 - 12536 http://pubs.acs.org/isubscribe/journals/jacsat/127/i36/figures/ja052120in00001.gif Abstract: We describe a novel approach to the rapid collection and processing of multidimensional NMR data: "high-resolution iterative frequency identification for NMR" (HIFI-NMR). As with other reduced dimensionality approaches, HIFI-NMR collects...	nmrlearner	Journal club	0	09-07-2005 08:21 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off