Question from NMRWiki Q&A forum topshim giving worse lineshape

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] topshim giving worse lineshape

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-10-2011, 08:52 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

topshim giving worse lineshape

topshim giving worse lineshape

HION bruker av-500 topshim giving worse line shape spectra for e.g it is getting the right keys but in wrong direction I mean it is adding too much in z3 and z5 but manualy it is nedded to minus.have any body any idea why it is?Thanks in advance for answering.

with best regardferoon

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Complete determination of the Pin1 catalytic domain thermodynamic cycle by NMR lineshape analysis. Complete determination of the Pin1 catalytic domain thermodynamic cycle by NMR lineshape analysis. Complete determination of the Pin1 catalytic domain thermodynamic cycle by NMR lineshape analysis. J Biomol NMR. 2011 Sep;51(1-2):21-34 Authors: Greenwood AI, Rogals MJ, De S, Lu KP, Kovrigin EL, Nicholson LK Abstract The phosphorylation-specific peptidyl-prolyl isomerase Pin1 catalyzes the isomerization of the peptide bond preceding a proline residue between cis and trans isomers. To best understand the mechanisms of Pin1 regulation,...	nmrlearner	Journal club	0	09-30-2011 05:59 AM
[NMRwiki tweet] nmrwiki: How to adjust topshim parameters? #nmr @bruker http://t.co/9lopPjA nmrwiki: How to adjust topshim parameters? #nmr @bruker http://t.co/9lopPjA nmrwiki: How to adjust topshim parameters? #nmr @bruker http://t.co/9lopPjA Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	07-25-2011 11:10 PM
[Question from NMRWiki Q&A forum] adjusting topshim parameters adjusting topshim parameters On our Avance III 500 we use Topshim quite successfully for any probe with a gradient coil. The only time we have a problem is with CDCl3 solvent on our 1.7 mm TXI probe, where Topshim fails with a complaint about the signal to noise ratio being too low. This is not surprising for a microprobe, as CDCl3 has the lowest signal of any common deuterated solvent. We have confirmed that the 2H pulse length is correct in the prosol file. An obvious solution would be to increase the nunber of scans for this probe/solvent combination. There seem to be probe and...	nmrlearner	News from other NMR forums	0	07-25-2011 11:01 AM
[NMR tweet] This chemistry is giving me a headache....why do I need to know about nuclear magnetic resonance spectroscopy This chemistry is giving me a headache....why do I need to know about nuclear magnetic resonance spectroscopy Published by stephanie_ayse (Stephanie Savory) on 2011-05-23T15:01:19Z Source: Twitter	nmrlearner	Twitter NMR	0	05-23-2011 03:02 PM
[NMR tweet] It's a shame robert winston isn't giving the machine its proper name of nuclear magnetic resonance imaging #missedopportunity It's a shame robert winston isn't giving the machine its proper name of nuclear magnetic resonance imaging #missedopportunity Published by simonjgray (simon gray) on 2010-12-23T20:22:12Z Source: Twitter	nmrlearner	Twitter NMR	0	12-23-2010 09:21 PM
[Stan NMR blog] Are NMR spectra worse today than they used to be? Are NMR spectra worse today than they used to be? A blog entry within the Know Thy Spins series More...	nmrlearner	News from NMR blogs	0	08-21-2010 05:42 PM
Programs for simulation of lineshape change due to chemical exchange http://www.shokhirev.com/nikolai/abc/nmrtut/ik1.gif 1) A simple web server for prediction of effect of chemical exchange on NMR lineshapes can be found here. 2) TwoLineNMR and TwoSiteExchange 3) MEXICO from Alex Bain group.	nmrlearner	NMR software	1	06-04-2009 05:54 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off