Hi, when I use the sp command with HNCO (and HNCACO) and HNCA (and HNCOCA) the strips are not contiguous since the C13 chemical shifts are very different. Is
[Question from NMRWiki Q&A forum] Why is HNCO the most sensitive Spectrum?
Why is HNCO the most sensitive Spectrum?
I would like to know where the advantages of the HNCO spectrum stem from. I read that it is the most sensitive multi-D NMR spectrum, but all i know is, that it produces an absorptive spectrum, which is an obvious advantage.
Can someone explain to me why it is the most sensitive 3D-NMR spectrum and why it is popular?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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04-17-2013 08:15 PM
[NMR Sparky Yahoo group] Re: Solid State Data and strip plots
Re: Solid State Data and strip plots
Hi Andrew, It might be difficult to get the Sparky spin graph assigner to do anything useful for your data. It was designed to create protein backbone walks
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nmrlearner
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05-20-2011 09:04 AM
[NMR Sparky Yahoo group] Solid State Data and strip plots
Solid State Data and strip plots
Hello all, I would like to make some strip plots in sparky using Solid-state data. While this may be done manually, I would very much prefer to use the spin
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nmrlearner
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05-17-2011 08:40 PM
HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment w
HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment
Abstract Described here is a set of three-dimensional (3D) NMR experiments that rely on CACA-TOCSY magnetization transfer via the weak
3 \textJ\textCa\textCa coupling. These pulse sequences, which resemble recently described 13C detected CACA-TOCSY (Takeuchi et al. 2010) experiments, are recorded in 1H2O, and use 1H excitation and detection. These experiments require alternate 13C-12C labeling together with perdeuteration,...
nmrlearner
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11-27-2010 07:34 PM
[NMR paper] 3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR expe
3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR experiments with two sequential connectivity pathways and high sensitivity.
Related Articles 3D TROSY-HNCA(coded)CB and TROSY-HNCA(coded)CO experiments: triple resonance NMR experiments with two sequential connectivity pathways and high sensitivity.
J Biomol NMR. 2004 Mar;28(3):289-94
Authors: Ritter C, Lührs T, Kwiatkowski W, Riek R
The concept of chemical shift-coding monitors chemical shifts in multi-dimensional NMR experiments without additional polarization...
nmrlearner
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11-24-2010 09:25 PM
[NMR paper] Structural analysis of silk with 13C NMR chemical shift contour plots.
Structural analysis of silk with 13C NMR chemical shift contour plots.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural analysis of silk with 13C NMR chemical shift contour plots.
Int J Biol Macromol. 1999 Mar-Apr;24(2-3):167-71
Authors: Asakura T, Iwadate M, Demura M, Williamson MP
The polymorphic structures of silk fibroins in the solid state were examined on the basis of a quantitative relationship between the 13C chemical shift and local structure in...
nmrlearner
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08-21-2010 04:03 PM
MQ-HNCO-TROSY for the measurement of scalar and residual dipolar couplings in larger
Abstract We describe a novel pulse sequence, MQ-HNCO-TROSY, for the measurement of scalar and residual dipolar couplings between amide proton and nitrogen in larger proteins. The experiment utilizes the whole 2TN polarization transfer delay for labeling of 15N chemical shift in a constant time manner, which efficiently doubles the attainable resolution in 15N dimension with respect to the conventional HNCO-TROSY experiment. In addition, the accordion principle is employed for measuring (J + D)NHs, and the multiplet components are selected with the generalized version of the TROSY scheme...
nmrlearner
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08-14-2010 04:19 AM
HNCO-based measurement of one-bond amide 15N-1H couplings with optimized precision
Abstract A pair of 3D HNCO-based experiments have been developed with the aim of optimizing the precision of measurement of 1JNH couplings. Both pulse sequences record 1JNH coupling evolution during the entire constant time interval that 15N magnetization is dephasing or rephasing with respect to the directly bonded 13Câ?² nucleus, with 15N13Câ?² multiple quantum coherence maintained during the 13Câ?² evolution period. The first experiment, designed for smaller proteins, produces an apparent doubling of the 1JNH coupling without any accompanying increases in line width. The second experiment...