Question from NMRWiki Q&A forum Strange peak distortions but that of water

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] Strange peak distortions but that of water

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-05-2013, 08:03 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Strange peak distortions but that of water

Strange peak distortions but that of water

Hy everyone, I'm having a strange problem when reading the NMR spectrum of a small aromatic molecule in deuterium oxide: all the resonances of the solute molecule are very distorted, as if caused by poor shimming. For example, they show multiple maxima, and are not symmetric. But the problem is that the HDO resonance has a perfectly symmetric Lorentzian lineshape. Could it be a shimming problem, even if the water resonance is perfect?

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Peak height versus peak volume Given a standard NOESY-based protein structure determination: Does anyone have any information on the benefits of measuring peak intensity by a volume integration method rather than simply measuring the peak height. Obviously integration is theoretically more accurate, but does it make any difference to the quality of the structures produced? especially if peak lineshapes are comparable? I was hoping to find some study comparing structures produced by both methods..... I'm also curious about the benefits of distance-calbrating NOEs to a curve rather than simply putting restraints...	paul	NMR Questions and Answers	3	09-15-2015 07:48 PM
[Question from NMRWiki Q&A forum] DOSY deep signal distortions DOSY deep signal distortions Hi heveryone,I'm trying to run some DOSY experiments, but the resulting spectra are VERY distorted, and this is not just a problem of phase. The strange thing is that these distortions didn't appear as long as a few days ago... Have you got any suggestion?Further details: The spectrometer is a Bruker 400MHz, and the sequence is the dstegp3s Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	06-26-2013 09:39 AM
[NMR Sparky Yahoo group] Peak picking Peak picking Dear all, I have a series of 3D spectra on a protein and now I need to pick the spectra. The usual way is to move across the planes for each spectrum but this More...	nmrlearner	News from other NMR forums	0	10-20-2012 06:28 PM
[NMR Sparky Yahoo group] Re: Peak picking Re: Peak picking 'kr' http://www.cgl.ucsf.edu/home/sparky/manual/extensions.html#RestrictedPick Keep your negative contours threshold very low to reduce artifacts in the More...	nmrlearner	News from other NMR forums	0	10-20-2012 06:28 PM
[NMRpipe Yahoo group] Peak Assignment in 2D Peak Assignment in 2D HI Everybody, Is it possible to assign my 2D NOESY data with help of already assigned pdb file using NMRPipe software. If yes,then how? thank you with regards More...	NMRpipe Yahoo group news	News from other NMR forums	0	07-30-2011 11:50 AM
Peak shape in NMR Hi there, I'm quite new to NMR, but one thing I've been wondering is: are the shapes of the peaks in an NMR spectrum important? I know that the frequency information is important, and the amplitude can sometimes be important for experiments like NOESY, but does the overall shape matter? Can anymore information be deduced from this? Thanks	newToNMR	NMR Questions and Answers	1	12-13-2010 06:50 AM
[NMR paper] Automated peak picking and peak integration in macromolecular NMR spectra using AUTOP Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY. Related Articles Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY. J Magn Reson. 1998 Dec;135(2):288-97 Authors: Koradi R, Billeter M, Engeli M, Güntert P, Wüthrich K A new approach for automated peak picking of multidimensional protein NMR spectra with strong overlap is introduced, which makes use of the program AUTOPSY (automated peak picking for NMR spectroscopy). The main elements of this program are a novel...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Refinement of the NMR solution structure of a protein to remove distortions arising f Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion. Related Articles Refinement of the NMR solution structure of a protein to remove distortions arising from neglect of internal motion. Biochemistry. 1991 Apr 23;30(16):3807-11 Authors: Fejzo J, Krezel AM, Westler WM, Macura S, Markley JL The effect of internal motion on the quality of a protein structure derived from nuclear magnetic resonance (NMR) cross relaxation has been investigated experimentally. Internal rotation of the...	nmrlearner	Journal club	0	08-21-2010 11:16 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off