Question from NMRWiki Q&A forum Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

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[Question from NMRWiki Q&A forum] Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

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NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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08-31-2015, 09:39 PM

nmrlearner

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Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

Splitting of amide peaks in indirect proton dimension of a 3D 15N Noesy spectra

Hello, I am trying to record 3D 15N Noesy-hsqc experiment on 15N labelled protein (50 a.a). The mixing time is 120 ms and the decoupler for 15N dimension is garp. Only the peaks in the amide region on the indirect proton dimension splitted into doublet with splitting ranging from 76-95 Hz. The 15N_HSQC spectra looks fine. What could be the possible reason for this? Please let me know your thoughts and inputs.

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