Hi, I'm working on a bruker avance 400 spectrometer and using topspin version 1.3 software. I want to get a std-nmr (saturation transfer difference nmr) spectrum. This is the first time for me to set up and processes a STD nmr experiment. I'm trying to use simplest pulse programme "stddiff" from brukers pulse programme catalogue. Could anyone help me abuot basic parameter settings (at least neccessary ones) for this experiment. For example I'm confused about "dimensions" of this püse programme. Because according to the pulse programme catalogue "stddiff" is a one dimensional, but when I tray this programme in accupars section and try to start accusition software tell me that "this programme is 2d or 3d so you have to change dimension". When I upgrade to the 2 dimension I get some spectrum but only x axis (f1 channel) have some useful information... and so on. Could anyone WHO have some experience abuot STD NMR experiments help me to get a simple STD spectrum please...
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[NMR images] where ‘brd’ refers to broadening, and all other parameters have ...
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where ‘brd’ refers to broadening, and all other parameters have ...
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Hi, comrades! We use the linux version of TopSpin 2.1 as a software for AVANCE II Bruker 400 MHz.As I found parameters of pulse sequences are located in /opt/topspin/exp/stan/nmr/par - directory.
I've started to study the content of "acqu" - file in directory /opt/topspin/exp/stan/nmr/par/PROTON and couldn't find any definitions of some parameters in Bruker-manuals. For instance, DBL, DATE, DBP, DBPNAME, DECBNUC, DECNUC, DECSTAT, DL, DP, DPO.
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nmrlearner
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[Question from NMRWiki Q&A forum] File with discription of parameters
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Hi, comrades! We use the linux version of TopSpin 2.1 as a software for AVANCE II Bruker 400 MHz.As I found parameters of pulse sequences are located in /opt/topspin/exp/stan/nmr/par - directory.
I've started to study the content of "acqu" - file in directory /opt/topspin/exp/stan/nmr/par/PROTON and couldn't find any definitions of some parameters in Bruker-manuals. For instance, DBL, DATE, DBP, DBPNAME, DECBNUC, DECNUC, DECSTAT, DL, DP, DPO.
Where can I find the meaning and explanation of that parameters?
nmrlearner
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Script to obtain order parameters from a structure
A Python script for prediction of order paramter from a structure is available from this website.
The script is based on the following paper
F. Zhang and R. Brüschweiler (2002) "Contact Model for the Prediction of NMR N-H Order Parameters in Globular Proteins" J. Am. Chem. Soc. 124(43), 12654-12655.
simple stddiff parameters
Linear Mode
