If I'm running relaxation experiments (15N) for R1, R2, and hetnoe on a double labeled (2H/15N) sample, do I need to do any deuterium decoupling added to the program?
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[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample
2DNOESY with N15 C13 Labelled sample or Unlabelled sample
Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ?
Thanks & Regards Srinivas
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11-01-2010 08:38 AM
[Question from NMRWiki Q&A forum] What has been done with direct detection of Deuterium?
What has been done with direct detection of Deuterium?
Hello, I am trying to investigate what has been done in NMR using direct detection of deuterium in liquids. - Could you suggest any citations?
What kind of sensitivity gain there is in using broadband probe vs. lock channel?
Has anything been done/can be done to detect scalar couplings with protons like in COSY or TOCSY? Are HD couplings other than geminal detectable?
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08-22-2010 02:30 AM
[NMRwiki tweet] nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled samp
nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/
nmrwiki: Should deuterium be decoupled in R2, R1 hetnoe expts for 2H/15N labeled sample? http://qa.nmrwiki.org/question/161/
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08-22-2010 01:49 AM
[U. of Ottawa NMR Facility Blog] Deuterium Decoupling
Deuterium Decoupling
Bruker NMR spectrometers equipped with a 2H TX board and 2H amplifier can be setup to carry out 2H observation and decoupling experiments while still maintaining a 2H lock. Two channel systems can use the 2H transmitter for 2H, 1H, 2H and X measurements. In addition to these, a three channel system can be set up for X measurements. The first figure below shows the 13C and 13C spectra collected for a mixture of deuterated solvents using two channels of an AVANCE spectrometer. The second figure shows the 13C, 13C, 13C and 13C spectra for the standard 13C sensitivity...
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08-21-2010 08:15 PM
[NMRpipe Yahoo group] Re: Deuterium referencing in conv.tcl
Re: Deuterium referencing in conv.tcl
Greetings all ... Alex is absolutely correct, and I get to wear the Deuterium Dunce Cap. We've updated and posted new versions of NMRPipe with this fix -- so,
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08-18-2010 11:15 AM
[NMRpipe Yahoo group] Deuterium referencing in conv.tcl
Deuterium referencing in conv.tcl
Hello, This isn't of immediate relevance to me, but I am curious nonetheless. I was looking through conv.tcl to learn a bit of how the varian and bruker
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08-18-2010 11:15 AM
Asking about Deuterium NMR
I wanted to do Deuterium NMR in human urine sample but no one has done previously in our lab.
In our Lab. we have 400 MHz Bruker (Avance) spectrometer with BroadBand Inverse probe (1H Observe, Z-gradient).
How we have to proceed without deuterium lock which is neccessory for exp. ?
Is above described instruments specification sufficient to perform the deutrium NMR experiments ?
Please any on can help
Thanks very much