Question from NMRWiki Q&A forum Setting up deuterium (2H) NMR on Bruker BBO probe

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[Question from NMRWiki Q&A forum] Setting up deuterium (2H) NMR on Bruker BBO probe

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NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

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GeNMR

I-TASSER

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Amber

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Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

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RCI

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HADDOCK

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Methyl S2

B-factor

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Shiftx2

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Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

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NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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09-17-2012, 02:13 PM

nmrlearner

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Setting up deuterium (2H) NMR on Bruker BBO probe

Setting up deuterium (2H) NMR on Bruker BBO probe

I need to set up 2H NMR on a Bruker 600 MHz BBO probe for the first time. Can anyone give me a set of instructions? Do I run in protonated or deuterated solvent? How do I tune 2H on the BB channel? What 2H values do I need in the edprosol table? What pulse program? What hardware connections or disconnections, e.g. removing the 2H stop filter....

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