What is meant by saturation transfer method in NMR? How it is useful in giving the structure of blue copper proteins like Azurin, Plastocyanin and Stellacyani? What is the need to go for this technique in blue copper proteins?
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[Question from NMRWiki Q&A forum] Can anyone help me with Saturation Transfer NMR
Can anyone help me with Saturation Transfer NMR
Hi I am working on a Varian 500MHz NMR. I am attempting to use the pulse sequence satxfer1D to measure the rate constant of an exchanging system. I am not using solvent suppression and I am having trouble understanding the physics behind the "mixing time" after the 90 pulse. I also do not know how many iterations the selective pulse should go through because it seems that if I turn the mixing time off and just use a selective pulse, and then a pw90, I will still obtain the same spectrum no matter how many iterations the selective pulse train...
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09-29-2011 07:36 PM
[U. of Ottawa NMR Facility Blog] Saturation Transfer and Exchange
Saturation Transfer and Exchange
Exchange processes that occur on the NMR time scale affect the NMR line shapes and can be studied by line shape analysis. If the exchange process is slow on the NMR time scale, one can employ EXSY or inversion transfer methods to study the exchange. An alternative to these is the saturation transfer technique. In this method, one of the slowly exchanging resonances (A) is saturated with low power CW irradiation and the effect on the intensity of the resonance of the exchange partner (B) is monitored. If there is exchange between A and B during the period of...
nmrlearner
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08-03-2011 01:00 AM
[NMR paper] Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experim
Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experiments to probe the binding of N-acetylneuraminic acid and derivatives to Vibrio cholerae sialidase.
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Proteins. 2004 Aug 1;56(2):346-53
Authors: Haselhorst T, Wilson JC, Thomson RJ, McAtamney S, Menting JG, Coppel RL, von Itzstein M
Saturation transfer difference...
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11-24-2010 10:01 PM
[NMR paper] SOS-NMR: a saturation transfer NMR-based method for determining the structures of pro
SOS-NMR: a saturation transfer NMR-based method for determining the structures of protein-ligand complexes.
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J Am Chem Soc. 2004 Mar 3;126(8):2390-8
Authors: Hajduk PJ, Mack JC, Olejniczak ET, Park C, Dandliker PJ, Beutel BA
An NMR-based alternative to traditional X-ray crystallography and NMR methods for structure-based drug design is described that enables the structure determination of ligands complexed to virtually...
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11-24-2010 09:25 PM
[NMR paper] Group epitope mapping by saturation transfer difference NMR to identify segments of a
Group epitope mapping by saturation transfer difference NMR to identify segments of a ligand in direct contact with a protein receptor.
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J Am Chem Soc. 2001 Jun 27;123(25):6108-17
Authors: Mayer M, Meyer B
A protocol based on saturation transfer difference (STD) NMR spectra was developed to characterize the binding interactions at an atom level, termed group epitope mapping (GEM). As an example...
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11-19-2010 08:32 PM
Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed int
Two-dimensional heteronuclear saturation transfer difference NMR reveals detailed integrin αvβ6 protein-peptide interactions.
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Chem Commun (Camb). 2010 Sep 13;
Authors: Wagstaff JL, Vallath S, Marshall JF, Williamson RA, Howard MJ
We report the first example of peptide-protein heteronuclear two-dimensional (2D) saturation transfer difference nuclear magnetic resonance (STD NMR). This method, resulting in...
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09-15-2010 02:26 PM
[NMR paper] 31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenin
31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenine nucleotide translocase.
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Biochemistry. 1991 Aug 27;30(34):8351-7
Authors: Masiakos PT, Williams GD, Berkich DA, Smith MB, LaNoue KF
The exchange of intramitochondrial ATP (ATP(in)) for extramitochondrial ATP (ATP(out)) was measured by using 31P NMR spectroscopy over a range of temperatures in isolated rat liver mitochondria oxidizing...
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08-21-2010 11:12 PM
[NMR paper] 31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenin
31P NMR saturation-transfer study of the in situ kinetics of the mitochondrial adenine nucleotide translocase.
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Biochemistry. 1991 Aug 27;30(34):8351-7
Authors: Masiakos PT, Williams GD, Berkich DA, Smith MB, LaNoue KF
The exchange of intramitochondrial ATP (ATP(in)) for extramitochondrial ATP (ATP(out)) was measured by using 31P NMR spectroscopy over a range of temperatures in isolated rat liver mitochondria oxidizing...
saturation transfer method in NMR
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