Dear sir/Madam, I am a sparky user and using this software for 2D NMR assignment....I used this software many time...I am facing a problem in ucsf file of 2D
[NMR Sparky Yahoo group] Re: sparky relaxation fit problem
Re: sparky relaxation fit problem
Hi mandar, Thanks. I have figure that out I used wrong unit for input. Tom ... From: Mandar T. Naik Subject: Re: sparky
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nmrlearner
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09-15-2012 09:04 AM
[NMR Sparky Yahoo group] Re: sparky relaxation fit problem
Re: sparky relaxation fit problem
Hi Tom, How are the fitted curves with respect to your data? You should inspect few by clicking on the 'rh' output table. I haven't crosschecked my results
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nmrlearner
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09-14-2012 07:54 AM
[NMR Sparky Yahoo group] sparky relaxation fit problem
sparky relaxation fit problem
Dear sparky users, I am trying to get r1 and R2 values I compared to nmrview. the results from sparky is 100 times smaller that the results I got from nmrview.
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nmrlearner
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09-14-2012 07:54 AM
[Question from NMRWiki Q&A forum] 3d Tocsy-hsqc mgnetization transfer problem
3d Tocsy-hsqc mgnetization transfer problem
Dear All,I acquired a 3d tocsy-hsqc edited on Bruker machine of small protein in micelles with a mixing time of 70ms. The problem is that I do not observe almoust spin system of each aminoacid, in detail I see only cross peaks between NH-HA. I obtained very good results by 3d Noesy-hsqc edited. How can I do to improve the magnetization transfer along the spin system and see all cross peaks? Do you think that this problem is due to the nature?Thank you in advance\
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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03-06-2012 06:17 PM
[Question from NMRWiki Q&A forum] Data processing and Sparky
Data processing and Sparky
Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions: 1. Our protein has about 132 residues.The NHSQC is well dispersed but many peaks can't be found in CBCANH and CBCACONH. HNCA/HNCOCA + HNCO/HNCACO are planned for backbone assignment. Other people processed data with NMRPipe. The NHSQC peaks can mostly find matching peaks in HNCO/HNCACO, while HNCA/HNCOCA can't be matched (no same resonances can be seen when synchronized). But, many peaks can be seen in HNCA/HNCOCA. I found that the whole scale of 13C axis in HNCA is from 51 to 66 and...
nmrlearner
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01-26-2012 02:22 PM
[NMR Sparky Yahoo group] Re: Sparky on Ubuntu 11.04 problem
Re: Sparky on Ubuntu 11.04 problem
Dear All, I found out later that I have to install the tcsh in order to have Sparky running properly. Thanks for the replys! Best
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nmrlearner
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05-17-2011 08:40 PM
[NMR Sparky Yahoo group] Re: Sparky on Ubuntu 11.04 problem
Re: Sparky on Ubuntu 11.04 problem
add the following line to your .bashrc export PATH=$PATH:/usr/local/sparky/bin good luck On Sat, May 14, 2011 at 12:51 PM, unclecheong2000 < ... -- Peng Ji
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nmrlearner
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05-16-2011 08:23 PM
[NMR Sparky Yahoo group] problem using autoassign in sparky
problem using autoassign in sparky
Dear all, I am not being able to understand how to use the 2D projection of 3D spectra for eg HNCA,HNCOCA etc for correcting my 3D spectra in sparky while
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