This is an example for an HN-Methyl 4D I have, not exactly the same, but perhaps useful to see anyway ... the processing scheme is a very basic one ...
[Question from NMRWiki Q&A forum] 2DNOESY with N15 C13 Labelled sample or Unlabelled sample
2DNOESY with N15 C13 Labelled sample or Unlabelled sample
Dear Friends, I am in process of doing structure calculation of dimeric protein 22kD by using ARIA2.2 . Can I acquire 2DNOESY spectrum for my protein. Will it provide useful information for structure calculation? And whether I should acquire 2DNOESY with N15 C13 Labelled sample or Unlabelled sample . Which sample would be appropriate for this kind of experiment on VNMRJ 700 with biopack ?
Thanks & Regards Srinivas
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nmrlearner
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02-06-2012 03:54 PM
[Question from NMRWiki Q&A forum] Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum
Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum
Dear friends,
I am in process of doing structure calculation of a dimeric protein. The problem I faced is regarding the assignment of N15Edit NOESY and C13 Edit NOESY spectrum.I have already processed the fid data and converted to sparky ucsf format. Is there options available in sparky for auto peak picking, integration and assignment of my NOESY spectrum? And second query is regarding the generation of input files for structure calculation. In our Lab we are using ARIA 2.1 software for structure calculation? Is there any...
nmrlearner
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12-14-2011 07:14 PM
[NMRpipe Yahoo group] Re: A sample 4D HCCH Noesy spectrum
Re: A sample 4D HCCH Noesy spectrum
Hi Have you tried the example from NMRDraw macro editor? (under process 4D) I believe nothing is special in term of processing data. Phasing, sweep width, and
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12-10-2011 02:38 AM
[NMRpipe Yahoo group] A sample 4D HCCH Noesy spectrum
A sample 4D HCCH Noesy spectrum
Hello, I'm struggling to process a 4D methyl noesy spectrum collected on a Bruker and I was wondering if anyone knows where I could find an example ser file
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12-10-2011 02:38 AM
[Question from NMRWiki Q&A forum] Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
I am facing problems in calibration of my HCCH-TOCSY spectrum in reference to HBHACONH and HCCCONH spectrum. Can somebody tell me how to solve this issue? Can I autoassign my HCCH-TOCSY spectrum using HBHACONH spectrum
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nmrlearner
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10-02-2011 08:25 AM
[Question from NMRWiki Q&A forum] HCCH-Tocsy connectivities
HCCH-Tocsy connectivities
What I see from this picture (linked from www.protein-nmr.org.uk):
http://www.protein-nmr.org.uk/pictures/experiment_types/hcch_tocsy.png
that shows an HCCH-TOCSY magnetisation transfer I do not see any transfer from HD to HB, but in my peaks I see a peak that seems it can be connected az Leu CB-HB2-HD22Can I have these peaks in HCCH-TOCSY?
would you please introduce me a reference that tell me what kind of connection can be visible in HC(C)H-Tocsy
nmrlearner
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11-17-2010 06:08 PM
[Question from NMRWiki Q&A forum] How many peaks should be in DOSY spectrum of D2O sample?
How many peaks should be in DOSY spectrum of D2O sample?
Hello, I've recorded a DOSY data set and processed with Bayesian DOSY transform. There seem to be at least two peaks in the processed spectrum. Is the second one an artifact?
In theory there might be a signal of H2O along with HDO, but wouldn't exchange of deuterium be much faster than the experiment time scale?
Thanks.
nmrlearner
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08-28-2010 10:31 AM
[Nature network NMR forum] HCCH-TOCSY (0 replies)
HCCH-TOCSY (0 replies)
Hello everybody,
I am a biologist who has jumped into NMR :(
I hope this is an active forum so that I can improve myself and can help someday to others.
I have a very stupid question!!! Do you know if I can see some HN peaks correlated with C-beta in HCCH-TOCSY?
Cheers,
Re: A sample 4D HCCH Noesy spectrum
Linear Mode
