Hi, His problem was in the nmrPipe processing script and has been resolved. According to me (after having seen his data), what you have suggested would not
[NMR Sparky Yahoo group] Re: Regarding processing problem of 2D TOCSY ucsf in sparky
Re: Regarding processing problem of 2D TOCSY ucsf in sparky
Hello Pancham, You can apply a chemical shift correction to your TOCSY spectrum using your ROESY spectrum as a reference: - First, do the peak picking of a few
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nmrlearner
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05-13-2013 11:36 AM
[NMR Sparky Yahoo group] Re: Regarding processing problem of 2D TOCSY ucsf in sparky
Re: Regarding processing problem of 2D TOCSY ucsf in sparky
Hi, Additionally, please post the output of ucsfdata program, which you can find in the bin folder of Sparky installation. Something like, ucsfdata TOCSY.ucsf
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nmrlearner
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05-10-2013 07:03 PM
[NMR Sparky Yahoo group] R: [nmr_sparky] Regarding processing problem of 2D TOCSY ucsf in spa
R: Regarding processing problem of 2D TOCSY ucsf in spa
Hi Pancham, did you check the acquisition parameters you used to record the spectrum? Best, Marco Dr.Marco Sette, Ph.D. Department of Chemical Sciences and
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nmrlearner
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05-10-2013 06:58 AM
[NMR Sparky Yahoo group] Regarding processing problem of 2D TOCSY ucsf in sparky
Regarding processing problem of 2D TOCSY ucsf in sparky
Dear sir/Madam, I am a sparky user and using this software for 2D NMR assignment....I used this software many time...I am facing a problem in ucsf file of 2D
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nmrlearner
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05-09-2013 05:37 PM
[NMR Sparky Yahoo group] Re: sparky relaxation fit problem
Re: sparky relaxation fit problem
Hi Kuen-Phon, You are correct. Thanks for help. Tom ... From: homespuner Subject: Re: sparky relaxation fit problem To:
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nmrlearner
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09-15-2012 09:04 AM
[NMR Sparky Yahoo group] Re: sparky relaxation fit problem
Re: sparky relaxation fit problem
Hi Tom, If I understand correctly, the unit of T2 in your case is mini-second (120 ms = 0.12 s). Therefore the R2 in your example is 1/0.12 = 8.33 s-1.
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nmrlearner
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09-14-2012 07:54 AM
[Question from NMRWiki Q&A forum] 3d Tocsy-hsqc mgnetization transfer problem
3d Tocsy-hsqc mgnetization transfer problem
Dear All,I acquired a 3d tocsy-hsqc edited on Bruker machine of small protein in micelles with a mixing time of 70ms. The problem is that I do not observe almoust spin system of each aminoacid, in detail I see only cross peaks between NH-HA. I obtained very good results by 3d Noesy-hsqc edited. How can I do to improve the magnetization transfer along the spin system and see all cross peaks? Do you think that this problem is due to the nature?Thank you in advance\
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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03-06-2012 06:17 PM
[Question from NMRWiki Q&A forum] Data processing and Sparky
Data processing and Sparky
Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions: 1. Our protein has about 132 residues.The NHSQC is well dispersed but many peaks can't be found in CBCANH and CBCACONH. HNCA/HNCOCA + HNCO/HNCACO are planned for backbone assignment. Other people processed data with NMRPipe. The NHSQC peaks can mostly find matching peaks in HNCO/HNCACO, while HNCA/HNCOCA can't be matched (no same resonances can be seen when synchronized). But, many peaks can be seen in HNCA/HNCOCA. I found that the whole scale of 13C axis in HNCA is from 51 to 66 and...