Hi Iris, It's difficult to diagnose the problem without seeing the processing script, but what phase correction was used in the 15N dimension? If the initial
[Question from NMRWiki Q&A forum] N15 NNH separated NOESY
N15 NNH separated NOESY
Dear friends,
I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get more information about this experiment from other sources. Is this experiment useful for providing restraints for protein structure calculation. what kind of information this experiment can provide and how to set up. If anyone has references to suggest, please suggest.
Thanks & Regards
Arun
nmrlearner
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02-21-2012 03:43 PM
[Question from NMRWiki Q&A forum] Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum
Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum
Dear friends,
I am in process of doing structure calculation of a dimeric protein. The problem I faced is regarding the assignment of N15Edit NOESY and C13 Edit NOESY spectrum.I have already processed the fid data and converted to sparky ucsf format. Is there options available in sparky for auto peak picking, integration and assignment of my NOESY spectrum? And second query is regarding the generation of input files for structure calculation. In our Lab we are using ARIA 2.1 software for structure calculation? Is there any...
nmrlearner
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12-14-2011 07:14 PM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY
Re: N15 separated NOESY and TOCSY
Thank you for your replies. The processing script of TOCSY (NH plane): $NMRTXT/ext.xz.com fid/test%03d.fid xz001.fid nmrPipe -in xz001.fid \ ... -out xzf.ft2
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05-29-2011 11:00 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY
Re: N15 separated NOESY and TOCSY
Hi Iris, Another question is what do you mean by the NH vs HH planes in regards to the 3D matrix (a single 3D peak can't be positive viewed along one axis and
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05-27-2011 10:40 AM
[NMRpipe Yahoo group] N15 separated NOESY and TOCSY
N15 separated NOESY and TOCSY
Hello, I did a N15 separated TOCSY experiment and used the following script: #!/bin/csh bruk2pipe -in ./ser \ -bad 0.0 -aswap -DMX -decim 2632 -dspfvs 20
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05-26-2011 10:34 PM
[Question from NMRWiki Q&A forum] 2D Filtered NOESY/TOCSY on Varian Biopack
2D Filtered NOESY/TOCSY on Varian Biopack
I am attempting to run some filtered NOESY and TOCSY experiments using sequences in the Varian Biopack - CNfiltocsy and CNfilnoesy. The experiments seem to run fine, but when overlaying the processed spectra something seems odd - the TOCSY crosspeaks are not present in the NOESY spectra and vice-versa. Does anyone know why this might be the case?
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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12-20-2010 01:52 AM
[U. of Ottawa NMR Facility Blog] Hsqc - tocsy
HSQC - TOCSY
There are many different NMR techniques used to probe molecular structure. Two very useful methods are the HSQC (or HMQC) sequence used to obtain heteronuclear coupling correlations and the TOCSY sequence used to look at extended homonuclear coupling correlations. These are among the most widely used pulse sequences by chemists to help elucidate the molecular structure of organic molecules. The sequences can also be combined into a single HSQC-TOCSY sequence which is simply an HSQC followed by a TOCSY spin lock. For the case of 1H and 13C in organic molecules, the HSQC-TOCSY...
nmrlearner
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08-21-2010 08:15 PM
1H-13C Separated Local Field Spectroscopy of Uniformly 13C Labeled Peptides and Prote
1H-13C Separated Local Field Spectroscopy of Uniformly 13C Labeled Peptides and Proteins
Publication year: 2010
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 1 July 2010</br>
Eugene C., Lin , Chin H., Wu , Yuan, Yang , Christopher V., Grant , Stanley J., Opella</br>
By incorporating homonuclear decoupling on both the 1H and 13C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with 13C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated...
Re: N15 separated NOESY and TOCSY
Linear Mode
