I once had a similar problem. In your case, the protein is fused so you know it is there. I suggest you get initial phases from the MBP molecular replacement
Improved technologies now routinely provide protein NMR structures useful for molecular replacement.
Improved technologies now routinely provide protein NMR structures useful for molecular replacement.
Improved technologies now routinely provide protein NMR structures useful for molecular replacement.
Structure. 2011 Jun 8;19(6):757-66
Authors: Mao B, Guan R, Montelione GT
Molecular replacement (MR) is widely used for addressing the phase problem in X-ray crystallography. Historically, crystallographers have had limited success using NMR structures as MR search models. Here, we report a comprehensive investigation of the utility of protein NMR...
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06-08-2011 11:30 AM
Alanine Methyl Groups as NMR Probes of Molecular Structure and Dynamics in High-Molecular-Weight Proteins
Alanine Methyl Groups as NMR Probes of Molecular Structure and Dynamics in High-Molecular-Weight Proteins
Raquel Godoy-Ruiz, Chenyun Guo and Vitali Tugarinov
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja1083656/aop/images/medium/ja-2010-083656_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/ja1083656
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/hxZ4cabF688
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12-08-2010 10:04 AM
[NMR paper] An expectation/maximization nuclear vector replacement algorithm for automated NMR re
An expectation/maximization nuclear vector replacement algorithm for automated NMR resonance assignments.
Related Articles An expectation/maximization nuclear vector replacement algorithm for automated NMR resonance assignments.
J Biomol NMR. 2004 Jun;29(2):111-38
Authors: Langmead CJ, Donald BR
We report an automated procedure for high-throughput NMR resonance assignment for a protein of known structure, or of an homologous structure. Our algorithm performs Nuclear Vector Replacement (NVR) by Expectation/Maximization (EM) to compute...
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11-24-2010 09:51 PM
[NMR paper] A polynomial-time nuclear vector replacement algorithm for automated NMR resonance as
A polynomial-time nuclear vector replacement algorithm for automated NMR resonance assignments.
Related Articles A polynomial-time nuclear vector replacement algorithm for automated NMR resonance assignments.
J Comput Biol. 2004;11(2-3):277-98
Authors: Langmead CJ, Yan A, Lilien R, Wang L, Donald BR
High-throughput NMR structural biology can play an important role in structural genomics. We report an automated procedure for high-throughput NMR resonance assignment for a protein of known structure, or of a homologous structure. These...
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11-24-2010 09:25 PM
[NMR paper] A systematic case study on using NMR models for molecular replacement: p53 tetrameriz
A systematic case study on using NMR models for molecular replacement: p53 tetramerization domain revisited.
Related Articles A systematic case study on using NMR models for molecular replacement: p53 tetramerization domain revisited.
Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1535-40
Authors: Chen YW, Clore GM
Molecular replacement using search models derived from nuclear magnetic resonance (NMR) spectroscopy has often proved problematic. It has been known for some time that the overall differences in atomic positions (r.m.s.d.)...
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11-19-2010 08:29 PM
[NMR paper] Does NMR mean "not for molecular replacement"? Using NMR-based search models to solve
Does NMR mean "not for molecular replacement"? Using NMR-based search models to solve protein crystal structures.
Related Articles Does NMR mean "not for molecular replacement"? Using NMR-based search models to solve protein crystal structures.
Structure. 2000 Nov 15;8(11):R213-20
Authors: Chen YW, Dodson EJ, Kleywegt GJ
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11-19-2010 08:29 PM
[NMR paper] Replacement of His23 by Cys in a zinc finger of HIV-1 NCp7 led to a change in 1H NMR-
Replacement of His23 by Cys in a zinc finger of HIV-1 NCp7 led to a change in 1H NMR-derived 3D structure and to a loss of biological activity.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Replacement of His23 by Cys in a zinc finger of HIV-1 NCp7 led to a change in 1H NMR-derived 3D structure and to a loss of biological activity.
FEBS Lett. 1993 Sep 27;331(1-2):43-8
Authors: Julian N, Demene H, Morellet N, Maigret B, Roques BP
The nucleocapsid protein NCp7 of human...
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08-22-2010 03:01 AM
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Re: Molecular replacement
Linear Mode
