Great suggestion! However, on our end ND0 is set to 1 for the SoFast HMQC and set to 2 for the regular HSQCs. So this does not seem to be the issue. Nicole
[NMRpipe Yahoo group] Re: Issues processing 2D SoFast HMQC
Re: Issues processing 2D SoFast HMQC
Jana, Despite ND0 being 1 for SoFast and 2 for regular HSQC, I decided to try your suggestion anyway. One more time processing this data set surely wouldn't
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09-06-2012 04:08 AM
[NMRpipe Yahoo group] Re: Issues processing 2D SoFast HMQC
Re: Issues processing 2D SoFast HMQC
Hi Nichole, Iam extremely sorry about the previous post. if you have mistakenly used nd0=2 for sfhmqc, try changing this line in the nmrPipe script from -xSW
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09-05-2012 03:35 AM
[NMRpipe Yahoo group] Re: Issues processing 2D SoFast HMQC
Re: Issues processing 2D SoFast HMQC
Hi Nicole, Pls check your sfhmqc experimental parameter 'nd0' ( using the command 'ased'). For sfhmqc the nd0 should be 1( for conventional HSQC nd0=2 ). If
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09-05-2012 03:35 AM
[NMRpipe Yahoo group] Re: Issues processing 2D SoFast HMQC
Re: Issues processing 2D SoFast HMQC
Hi Jana, I'm sorry, I should have been more clear in my post. I do use States-TPPI when processing the SoFast data. (I have also tried a plethora of other
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09-05-2012 03:35 AM
[NMRpipe Yahoo group] Re: Issues processing 2D SoFast HMQC
Re: Issues processing 2D SoFast HMQC
Hi Nicole, The yMode is not Echo-AntiEcho for the ppg sfhmqcf3gpph, it should be -yMODE States-TPPI Pls check the first line:- -bad 0.0 -noaswap -DMX -decim
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09-05-2012 03:35 AM
[NMRpipe Yahoo group] Issues processing 2D SoFast HMQC
Issues processing 2D SoFast HMQC
Hello all, I am experiencing a problem similar to one posted about in June 2008 (message ID: 1752), only the solutions given then aren't working for me.
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09-05-2012 03:35 AM
[U. of Ottawa NMR Facility Blog] 19f - 13c hmqc
19F - 13C HMQC
If one has an NMR spectrometer with hardware capable of synthesizing and amplifying the frequency of 19F from the 1H channel and a broadband NMR probe whose 1H channel can tune down to 19F, then one is able to do 19F - 13C HMQC experiments. The figure below shows an example of a 19F - 13C HMQC spectrum collected on a Bruker AVANCE 500 NMR spectrometer using a 5 mm broadband probe. The fluorine spectrum is plotted on the top and the 13C spectrum is plotted on the side. The panel on the left shows the spectrum optimized for one-bond coupling, while that on the right shows the...
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08-21-2010 08:15 PM
15N SOFAST-HMQC to study fast H-D exchange
Very Fast Two-Dimensional NMR Spectroscopy for Real-Time Investigation of Dynamic Events in Proteins on the Time Scale of Seconds
Paul Schanda and Bernhard Brutscher
J. Am. Chem. Soc.; 2005; 127(22) pp 8014 - 8015
http://pubs.acs.org/isubscribe/journals/jacsat/127/i22/figures/ja051306en00001.gif
Abstract:
We demonstrate for different protein samples that 2D 1H-15N correlation NMR spectra can be recorded in a few seconds of acquisition time using a new band-selective optimized flip-angle short-transient heteronuclear multiple quantum coherence experiment. This has enabled us to...