Ronald and other Pipers, My group has developed an interactive GUI-based software package for analysis of dispersion data that you may find useful, named
Quantifying Millisecond Exchange Dynamics in Proteins by CPMG Relaxation Dispersion NMR Using Side-Chain 1H Probes
Quantifying Millisecond Exchange Dynamics in Proteins by CPMG Relaxation Dispersion NMR Using Side-Chain 1H Probes
Alexandar L. Hansen, Patrik Lundstrom, Algirdas Velyvis and Lewis E. Kay
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja210711v/aop/images/medium/ja-2011-10711v_0008.gif
Journal of the American Chemical Society
DOI: 10.1021/ja210711v
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/jaMjjnA_QTw
nmrlearner
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02-03-2012 09:50 AM
[NMRpipe Yahoo group] Re: CPMG / Relaxation-Dispersion
Re: CPMG / Relaxation-Dispersion
These are all good comments, many thanks to my fellow Pipers for discussing Ronald's question. Note also, it's possible to extract evolution curves from
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11-22-2011 09:52 AM
[NMRpipe Yahoo group] Re: CPMG / Relaxation-Dispersion
Re: CPMG / Relaxation-Dispersion
Hi Ronald, I am not sure NMRpipe has the routine to do relaxation measurements/analysis. I use Sparky to analyze my CPMG data. In sparky open multiple spectra
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11-22-2011 09:52 AM
[NMRpipe Yahoo group] Re: CPMG / Relaxation-Dispersion
Re: CPMG / Relaxation-Dispersion
Hi Ronald - There are three steps to the analysis of such curves: determining the intensity of each peak, converting those to an apparent R2 value for each
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11-21-2011 08:57 PM
[NMRpipe Yahoo group] CPMG / Relaxation-Dispersion
CPMG / Relaxation-Dispersion
Dear NMRPipers, Does the NMRPipe software have the ability to do CPMG Relaxation-Dispersion analysis? I just finished running a pseudo-3D experiment on our
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11-21-2011 08:57 PM
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups
Abstract A new pulse sequence is presented for the measurement of relaxation dispersion profiles quantifying millisecond time-scale exchange dynamics of side-chain carbonyl groups in uniformly 13C labeled proteins. The methodology has been tested using the 87-residue colicin E7 immunity protein, Im7, which is known to fold via a partially structured low populated intermediate that interconverts with the folded, ground state on the millisecond time-scale....
nmrlearner
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06-20-2011 03:31 PM
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups.
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups.
Quantifying millisecond time-scale exchange in proteins by CPMG relaxation dispersion NMR spectroscopy of side-chain carbonyl groups.
J Biomol NMR. 2011 Jun 18;
Authors: Hansen AL, Kay LE
A new pulse sequence is presented for the measurement of relaxation dispersion profiles quantifying millisecond time-scale exchange dynamics of side-chain carbonyl groups in uniformly (13)C labeled proteins. The methodology has...
Re: CPMG / Relaxation-Dispersion
Linear Mode
