I have seen many different protocols on making acrylamide gels for RDC measurements which include usually one of the following 1)drying the gel and re-swelling with protein solution2)allowing equilibration of protein into an already swelled gel
It seems as though you can get a much higher protein conc. if you dry the gel so what is the advantage to letting it equilibrate?
Thanks
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<!-- start content --> <table style="width: 76px; height: 63px;" id="toc" class="toc" summary="Contents"><tbody><tr><td>Contents
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Sample preparation
Sample preparation Samples
The Samples program is designed to collect and store some essential information pertaining to the samples used to record the spectra.
Residual Dipolar Couplings
Bicelle preparation notes from Avanti
Instructions for bicelle preparation from Avanti
RDC gel preparation
Linear Mode
