Hi i'm a newbie in NMR and I have 2 proteins, A protein is about 12KDa, B protein is about 45 KDa,i know that A protein binds ssRNA 20nucleotide molecule. My question what is the best way to know if protein B forms a complex A and RNA using NRM techniques? I have thought of deutering protein B, and mapping chemical shifts in isotopic labelled (15N) A protein, is this ok?... can transfered NOE or filtered NOE be used?? what about PRE? I've heard of TROSY, how can this help me?
Thanks for your ideas. Structural biology Master student
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NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions.
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NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions.
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Protein-protein interactions are crucial for a wide variety of biological processes. These interactions range from high affinity (K (d) < nM) to very low affinity (K (d) > mM). While much is known about the nature of high affinity protein complexes, our knowledge about structural characteristics...
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Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR/x-ray crystallography
Spincore.com are advertising a postdoc NMR position. It sounds pretty interesting.
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Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR /x-ray crystallography
Case Medical School, Cleveland, Ohio, USA
How are signaling events transmitted from one protein to another? To answer this question we are looking to add a postdoctoral co-workers to our interdisciplinary team. Our interest is to understand protein-protein interactions, protein structure and dynamics in the context of cell signaling...
Protein-Protein Interactions
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