Hi everyone! I’m working with a intregal membrane protein over-expresed in human cell lines. My principal problem is to find the optimal conditions for solubilization, I know that my protein is very unstable. Recently I read the paper from Ericcson and colleages about the thermofluor Assay. Despite the low sensibility due to the detergents I need to solubilizate my protein I think the assay will work correctly if I can configure the thermal cicler. I’m using the Bio-Rad MyiQ real time PCR system, but I can’t configure it to correctly read Sypro Orange fluorescence, Anyone has used it? Thanks
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Cracking the molecular weight barrier: Fragment screening of an aminotransferase using an NMR-based functional assay.
Bioorg Med Chem Lett. 2011 Jul 21;
Authors: Mendoza R, Petros AM, Liu Y, Thimmapaya R, Surowy CS, Leise WF, Pereda-Lopez A, Panchal SC, Sun C
NMR-based screening of protein targets has become a well established part of the drug discovery process especially with respect to fragments. However, as target size increases...
nmrlearner
Journal club
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08-16-2011 01:19 PM
[Nature network NMR forum] structure determination (0 replies)
structure determination (0 replies)
Hi every body,
My problem is to predict the structure of unknown compound by using spectral analysis; NMR, IR, UV, Mass spectra and elemental analysis.
Can anyone help me?
For help, contact me on my mail; m.fafy@yahoo.com.
nmrlearner
News from other NMR forums
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03-15-2011 05:56 AM
[NMR paper] Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein r
Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein receptor target in the natural membrane environment: electronic structure of the retinylidene chromophore in rhodopsin.
Related Articles Ultra-high-field MAS NMR assay of a multispin labeled ligand bound to its G-protein receptor target in the natural membrane environment: electronic structure of the retinylidene chromophore in rhodopsin.
Biochemistry. 2001 Mar 20;40(11):3282-8
Authors: Verhoeven MA, Creemers AF, Bovee-Geurts PH, De Grip WJ, Lugtenburg J, de Groot HJ
...
nmrlearner
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11-19-2010 08:32 PM
[NMR paper] An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Related Articles An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Kurume Med J. 1990;37(3):153-7
Authors: Chinami M, Shingu M
The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial...
nmrlearner
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08-21-2010 10:48 PM
[Nature network NMR forum] NMR and MRI community (0 replies)
NMR and MRI community (0 replies)
Hi ALl
Here is a Chinese NMR, MRI and EPR community. Most are in Chinese, some in English.
http://bbs.chinanmr.cn
the Chinese NMR wiki http://wiki.chinanmr.cn
Thanks.
nmrlearner
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08-21-2010 03:29 PM
[Nature network NMR forum] ResearchGATE (3 replies)
ResearchGATE (3 replies)
ResearchGATE is a new internet resource for connecting scientists and helping to develop collaborations and share resources and data. They have even promised a biomolecular NMR group!
Visit and sign up here
More...
nmrlearner
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08-21-2010 03:29 PM
[Nature network NMR forum] NMR Wiki (0 replies)
NMR Wiki (0 replies)
Hello,
I’ve just found this forum and decided to post a message about NMR Wiki (http://nmrwiki.org) – The Open NMR Project.
Users of NMR Wiki can:
*search and update the pulse sequence database
*create pulse sequence images from wiki text
*read and write about theory and practice of spectroscopy, your software and anything else relevant to Magnetic Resonance
*insert and cite bibliography in the style of real papers
nmrlearner
News from other NMR forums
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08-21-2010 03:29 PM
[Nature network NMR forum] NMR in plasma (6 replies)
NMR in plasma (6 replies)
In a previous post on this forum I mentioned that there were some neat methods for doing in-cell nmr.
I have been wondering since then whether any of you had tried just adding stable-isotope-labeled proteins to plasma. Would the results of such an experiment be interesting?
If you know of a paper that describes the results of this, would you please let me know – my PubMed searches have not been fruitful so far.
Protein NMR: The Thermofluor Assay (0 replies)
Linear Mode
