Hi, Is there a way to process Bruker 2D spectra acquired with QF in t1 and end up with a phase-sensitive spectrum instead of a magnitude spectrum? I am trying
[Question from NMRWiki Q&A forum] Data processing and Sparky
Data processing and Sparky
Dear NMRWikiers, I'm fresh in nmr field and would like to ask couple questions: 1. Our protein has about 132 residues.The NHSQC is well dispersed but many peaks can't be found in CBCANH and CBCACONH. HNCA/HNCOCA + HNCO/HNCACO are planned for backbone assignment. Other people processed data with NMRPipe. The NHSQC peaks can mostly find matching peaks in HNCO/HNCACO, while HNCA/HNCOCA can't be matched (no same resonances can be seen when synchronized). But, many peaks can be seen in HNCA/HNCOCA. I found that the whole scale of 13C axis in HNCA is from 51 to 66 and...
nmrlearner
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01-26-2012 02:22 PM
[Question from NMRWiki Q&A forum] data processing
data processing
HELLO NMR WIKI ERS
Could you please give information about the standred reference values of LINEAR PRIDICTION FORWRAD or BACKWORD during the processing of 3D data NMR DATA and when the sinebell shift value is needed and which wieghting function Shall i use it everytime to get good contours or signal on VNMRJ software
Thanking You in anticipation
Sri
nmrlearner
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05-13-2011 07:41 AM
[NMRpipe Yahoo group] difficulty in processing data
difficulty in processing data
Hello All, I am a new user to nmrpipe...I am able to execute the commands for processing 2D data but then I am unsure if I have correctly processed it or
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12-15-2010 12:16 AM
[NMRpipe Yahoo group] processing 2D data
processing 2D data
Hi all, 1. How to get stack plots in nmrPipe. 2. I have to compare volumes of different 2D spectra. But, for each spectra different noise levels i am getting.
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12-02-2010 08:41 AM
[NMRpipe Yahoo group] Re: processing 2D data
Re: processing 2D data
... I think that Bruker's definition of phase values is different than NMRPipe's. Specifically, NMRPipe's phase values are the negative of the Bruker ones.
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11-03-2010 11:27 AM
[NMRpipe Yahoo group] processing 2D data
processing 2D data
Hi NMRPipe users, I am yasin new user of nmrpipe. I have few problems while processing the 2D data to pipe. 1> when topspin 2.1 phase values kept in nmrpipe we
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11-03-2010 11:27 AM
[Question from NMRWiki Q&A forum] data processing by nmrPipe
data processing by nmrPipe
Hi all,I am very new to nmrPipe. I want to achieve the following steps. It would be very kind if somebody helps.(1) I have a 2D bruker data (t2= 4k points, t1= 32 points) and I want to do Fourier transform in the F2 dimension,phase etc. (2) and then extract the 32 spectra as 1Ds and also convert these 32 spectra in ascii format.(3) fit the peaks with a gaussian or Lorentzian function.(4) integrate the peak and save the integrals and time points as an ascii file.I only managed till the 1st step.Please help..!!
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nmrlearner
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10-05-2010 02:11 PM
Broadband homonuclear TOCSY with amplitude and phase-modulated RF mixing schemes
Broadband homonuclear TOCSY with amplitude and phase-modulated RF mixing schemes
Anika Kirschstein, Christian Herbst, Kerstin Riedel, Michela Carella, Jörg Leppert, Oliver Ohlenschläger, Matthias Görlach and Ramadurai Ramachandran
Journal of Biomolecular NMR; 2008; 40(4); pp 227-237
Abstract:
We have explored the design of broadband scalar coupling mediated 13C–13C and cross-relaxation suppressed 1H–1H TOCSY sequences employing phase/amplitude modulated inversion pulses. Considering a variety of supercycles, pulsewidths and a RF field strength of 10 kHz, the Fourier coefficients...
processing phase-modulated Bruker QF data
Linear Mode
