Question from NMRWiki Q&A forum Problem with the gradient shimming

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] Problem with the gradient shimming

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

10-11-2011, 06:35 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,700

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Problem with the gradient shimming

Problem with the gradient shimming

Dear Friends,

I've recently encountered a problem with gradient shimming that has never happened to me before. I can't clearly determine what caused it but it certainly didn't involve anybody manipulating with the probe. Here's the symptom: when running the gmapz experiment, the Z-profiles (before and after gradient) look like this:

As you can see the second profile (after the gradient) is distorted at the left edge (more or less severely, depending on a sample - here it's just a tiny "step"). I assume it has something to do with the transverse shims - but I'm not really sure which of them I am supposed to modify. Maybe some of you have experienced this kind of behaviour?

Here's a shimmap I've made using 10% D2O:

I wonder if this distortion influences my gradient experiments (which use only axial gradient)? 2D spectra seem to be just fine but I'm afraid of the results from diffusion ordered experiments. I'll appreciate any help! Thank you in advance!

D. Kubicki

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
solution to a basic nmr problem, practice problem 3 solution to a basic nmr problem, practice problem 3 http://i.ytimg.com/vi/mOdABDblqKo/default.jpg solution to a basic nmr problem, practice problem 3 The solution to a good, basic practice nmr and IR problem at Bryn Mawr College by M Nerz From: mnerzsto Views: 4060 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif ...	nmrlearner	NMR educational videos	0	09-14-2011 09:34 PM
[NMRwiki tweet] nmrwiki: How to turn ON fid for shimming in VNMRJ? #nmrhttp://qa.nmrwiki.org/question/231/problems-about-vnmrj-23a-1shimming nmrwiki: How to turn ON fid for shimming in VNMRJ? #nmrhttp://qa.nmrwiki.org/question/231/problems-about-vnmrj-23a-1shimming nmrwiki: How to turn ON fid for shimming in VNMRJ? #nmrhttp://qa.nmrwiki.org/question/231/problems-about-vnmrj-23a-1shimming Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	03-04-2011 02:12 AM
[Question from NMRWiki Q&A forum] Problems about VnmrJ 2.3A (1.shimming) Problems about VnmrJ 2.3A (1.shimming) when shimming with Fid scan, the fid/spectrum can't be displayed in real time. Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	02-27-2011 01:30 PM
[Question from NMRWiki Q&A forum] gradient shim map question gradient shim map question Does anyone know for sure if a shim map is off center slightly left in a negative direction...and I assume it is a centering issue do you whether it would be probe up/down? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	10-19-2010 06:35 PM
Gradient Coils http://farm4.static.flickr.com/3304/3268388270_9f03dd66e6.jpg Gradient Coils More...	nmrlearner	NMR pictures	0	08-18-2010 01:12 PM
shimming Hello Dose any one can estimate how are the results of shimming depend on the spectral character of the shim standard ? (for any NMR system) For example using single peak vs 2 peak material such as water or xylene Thanks	oferv	NMR Questions and Answers	0	02-22-2007 12:06 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off