Question from NMRWiki Q&A forum poor signal to noise on 1H-15N heteronculear NOE

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[Question from NMRWiki Q&A forum] poor signal to noise on 1H-15N heteronculear NOE

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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11-01-2011, 01:52 AM

nmrlearner

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poor signal to noise on 1H-15N heteronculear NOE

poor signal to noise on 1H-15N heteronculear NOE

I have recently attempted to run a 1H-15N heteronuclear NOE experiment in vnmrj (700 rt probe) using the standard sequence that comes with biopack, the signal to nose im getting compared to my standard HSQC is significantly reduced, is this normal? I do not recall this being the case when I ran this previously on a bruker 600 with RT probe? is this normal or does this suggest something is miss set? Also in the no NOE experiment I get very broad noise in the centre of my spectrum/water line.

regards

Tom

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