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[Question from NMRWiki Q&A forum] peak missing in nh hsqc protein nmr

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NMR processing:

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06-28-2011, 04:40 PM

nmrlearner

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peak missing in nh hsqc protein nmr

peak missing in nh hsqc protein nmr

Dear NMR wikiers

iam assigning 95 aminoacid residue protein . i can see 76 cross peaks in hsqc on 700 vnmr cryob . still some peaks are missing . i tried at modified ph conditions , buffer ionic strength and d2o percentage ( 5 to 10 percent ).but no significant improvement

is their any experment to get best number of cross peaks in hsqc ?

Check if somebody has answered this question on NMRWiki QA forum

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