Greetings all, Long-time reader, first-time poster. I am attempting to use nlinLS to curve-fit and extract peak intensities across a series of HSQC spectra. My
Peak height versus peak volume
Given a standard NOESY-based protein structure determination: Does anyone have any information on the benefits of measuring peak intensity by a volume integration method rather than simply measuring the peak height.
Obviously integration is theoretically more accurate, but does it make any difference to the quality of the structures produced? especially if peak lineshapes are comparable?
I was hoping to find some study comparing structures produced by both methods.....
I'm also curious about the benefits of distance-calbrating NOEs to a curve rather than simply putting restraints...
paul
NMR Questions and Answers
3
09-15-2015 07:48 PM
Unequal quadrupolar splitting intensities
Hi everyone
I'm running an experiment looking at a mesoporous organosilica soaked in benzene-d6. The pores are tubular and aligned more or less in the same direction, so the anisotropic environment causes quadrupolar splitting when looking at deuterium. However, the splitting intensities are not equal: the left peak tends to be noticeably larger (~10-20%) than the right. Have tried this with the sample at different orientations in the field. At some angles, such at 180 degrees, the peaks are nearly equal in intensity, but at the rest of them the left is higher.
So my question is: what...
sir_manning
NMR Questions and Answers
0
02-06-2012 06:33 PM
Measurement of 1Hâ??15N and 1Hâ??13C residual dipolar couplings in nucleic acids from TROSY intensities
Measurement of 1Hâ??15N and 1Hâ??13C residual dipolar couplings in nucleic acids from TROSY intensities
Abstract Analogous to the recently introduced ARTSY method for measurement of one-bond 1Hâ??15N residual dipolar couplings (RDCs) in large perdeuterated proteins, we introduce methods for measurement of base 13Câ??1H and 15Nâ??1H RDCs in protonated nucleic acids. Measurements are based on quantitative analysis of intensities in 1Hâ??15N and 13Câ??1H TROSY-HSQC spectra, and are illustrated for a 71-nucleotide adenine riboswitch. Results compare favorably with those of conventional...
nmrlearner
Journal club
0
09-30-2011 08:01 PM
[NMRpipe Yahoo group] Peak Assignment in 2D
Peak Assignment in 2D
HI Everybody, Is it possible to assign my 2D NOESY data with help of already assigned pdb file using NMRPipe software. If yes,then how? thank you with regards
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NMRpipe Yahoo group news
News from other NMR forums
0
07-30-2011 11:50 AM
[NMR paper] Automated peak picking and peak integration in macromolecular NMR spectra using AUTOP
Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
Related Articles Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
J Magn Reson. 1998 Dec;135(2):288-97
Authors: Koradi R, Billeter M, Engeli M, Güntert P, Wüthrich K
A new approach for automated peak picking of multidimensional protein NMR spectra with strong overlap is introduced, which makes use of the program AUTOPSY (automated peak picking for NMR spectroscopy). The main elements of this program are a novel...
nmrlearner
Journal club
0
11-17-2010 11:15 PM
[NMR Sparky Yahoo group] Re: peak color
Re: peak color
I will give it a shot, but there is now way to edit some code that comes with MacOS version? ________________________________ From: Tom Goddard
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nmrlearner
News from other NMR forums
0
11-10-2010 04:10 AM
Carbene peak 13C NMR.png
http://upload.wikimedia.org/wikimedia/commons/thumb/7/7e/Carbene_peak_13C_NMR.png/282px-Carbene_peak_13C_NMR.png
Uploaded by user "Quantockgoblin" on Sat, 22 Mar 2008 01:53:00 UTC
Added to category on Mon, 21 Sep 2009 15:35:30 UTC
Original image: 440×468 pixel; 24.143 bytes.
Licensing : Public domain
Carbene peak 13C NMR.png
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nmrlearner
NMR pictures
0
11-01-2010 08:38 AM
[NMRpipe Yahoo group] Re: Peak intensities from nlinLS
Re: Peak intensities from nlinLS
Greetings Joshua and all Dear Pipers, In the peak series model, the values which are adjusted by the fit are HEIGHT XW YW X_AXIS Y_AXIS and Z_A1 etc. Z_A0 is
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Peak intensities from nlinLS
Linear Mode
