Peak height versus peak volume
Given a standard NOESY-based protein structure determination: Does anyone have any information on the benefits of measuring peak intensity by a volume integration method rather than simply measuring the peak height.
Obviously integration is theoretically more accurate, but does it make any difference to the quality of the structures produced? especially if peak lineshapes are comparable?
I was hoping to find some study comparing structures produced by both methods.....
I'm also curious about the benefits of distance-calbrating NOEs to a curve rather than simply putting restraints...
paul
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09-15-2015 07:48 PM
[NMRpipe Yahoo group] Re: color combination in nmrpipe
Re: color combination in nmrpipe
Hi Amretia Sounds like you have negative and positive peaks in a 2D spectra, you can check by changing your contour level for the negative peaks and see if the
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05-27-2011 10:40 AM
[NMR Sparky Yahoo group] Re: color code in sparky
Re: color code in sparky
If you are expecting only positive intensity (red) peaks in your spectrum, make sure that the green (negative intensity) peaks that you are seeing are much
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nmrlearner
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12-08-2010 10:04 AM
[NMR Sparky Yahoo group] color code in sparky
color code in sparky
Dear All, We are new user of sparky, we have read in sparky manual that after processing the time-doamin data from NMRPipe we can import its output in sparky
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nmrlearner
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12-07-2010 09:54 AM
[NMRpipe Yahoo group] color combination in nmrpipe
color combination in nmrpipe
Dear all, We are new user of nmrpipe, while doing the 2D processing we are getting peaks with two colors but we don't know which one we have to consider for
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12-06-2010 09:44 PM
[NMR paper] Automated peak picking and peak integration in macromolecular NMR spectra using AUTOP
Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
Related Articles Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
J Magn Reson. 1998 Dec;135(2):288-97
Authors: Koradi R, Billeter M, Engeli M, Güntert P, Wüthrich K
A new approach for automated peak picking of multidimensional protein NMR spectra with strong overlap is introduced, which makes use of the program AUTOPSY (automated peak picking for NMR spectroscopy). The main elements of this program are a novel...
nmrlearner
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11-17-2010 11:15 PM
[NMR Sparky Yahoo group] Re: peak color
Re: peak color
I will give it a shot, but there is now way to edit some code that comes with MacOS version? ________________________________ From: Tom Goddard
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nmrlearner
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11-10-2010 04:10 AM
[NMR Sparky Yahoo group] Re: peak color
Re: peak color
Hi Andrew, Unfortunately the white color of the peak markers and labels in Sparky is coded into the C++ and cannot be changed without recompiling Sparky. If
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