HI Everybody, Is it possible to assign my 2D NOESY data with help of already assigned pdb file using NMRPipe software. If yes,then how? thank you with regards
Peak height versus peak volume
Given a standard NOESY-based protein structure determination: Does anyone have any information on the benefits of measuring peak intensity by a volume integration method rather than simply measuring the peak height.
Obviously integration is theoretically more accurate, but does it make any difference to the quality of the structures produced? especially if peak lineshapes are comparable?
I was hoping to find some study comparing structures produced by both methods.....
I'm also curious about the benefits of distance-calbrating NOEs to a curve rather than simply putting restraints...
paul
NMR Questions and Answers
3
09-15-2015 07:48 PM
Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data
Protein side-chain resonance assignment and NOE assignment using RDC-defined backbones without TOCSY data
Abstract One bottleneck in NMR structure determination lies in the laborious and time-consuming process of side-chain resonance and NOE assignments. Compared to the well-studied backbone resonance assignment problem, automated side-chain resonance and NOE assignments are relatively less explored. Most NOE assignment algorithms require nearly complete side-chain resonance assignments from a series of through-bond experiments such as HCCH-TOCSY or HCCCONH. Unfortunately, these TOCSY...
nmrlearner
Journal club
0
06-27-2011 04:30 AM
[NMR paper] More reliable protein NMR peak assignment via improved 2-interval scheduling.
More reliable protein NMR peak assignment via improved 2-interval scheduling.
Related Articles More reliable protein NMR peak assignment via improved 2-interval scheduling.
J Comput Biol. 2005 Mar;12(2):129-46
Authors: Chen ZZ, Lin G, Rizzi R, Wen J, Xu D, Xu Y, Jiang T
Protein NMR peak assignment refers to the process of assigning a group of "spin systems" obtained experimentally to a protein sequence of amino acids. The automation of this process is still an unsolved and challenging problem in NMR protein structure determination. Recently,...
nmrlearner
Journal club
0
11-24-2010 11:14 PM
[NMR paper] Automated peak picking and peak integration in macromolecular NMR spectra using AUTOP
Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
Related Articles Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
J Magn Reson. 1998 Dec;135(2):288-97
Authors: Koradi R, Billeter M, Engeli M, Güntert P, Wüthrich K
A new approach for automated peak picking of multidimensional protein NMR spectra with strong overlap is introduced, which makes use of the program AUTOPSY (automated peak picking for NMR spectroscopy). The main elements of this program are a novel...
nmrlearner
Journal club
0
11-17-2010 11:15 PM
[NMR Sparky Yahoo group] Re: peak color
Re: peak color
I will give it a shot, but there is now way to edit some code that comes with MacOS version? ________________________________ From: Tom Goddard
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nmrlearner
News from other NMR forums
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11-10-2010 04:10 AM
Carbene peak 13C NMR.png
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Carbene peak 13C NMR.png
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nmrlearner
NMR pictures
0
11-01-2010 08:38 AM
[NMR Sparky Yahoo group] peak color
peak color
Is there a way to edit the some file so that every time i pick a peak and label it so that they come up black instead of white?
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Peak Assignment in 2D
Linear Mode
