In a previous post on this forum I mentioned that there were some neat methods for doing in-cell nmr.
I have been wondering since then whether any of you had tried just adding stable-isotope-labeled proteins to plasma. Would the results of such an experiment be interesting?
If you know of a paper that describes the results of this, would you please let me know – my PubMed searches have not been fruitful so far.
[NMR paper] PMP1 18-38, a yeast plasma membrane protein fragment, binds phosphatidylserine from b
PMP1 18-38, a yeast plasma membrane protein fragment, binds phosphatidylserine from bilayer mixtures with phosphatidylcholine: a (2)H-NMR study.
Related Articles PMP1 18-38, a yeast plasma membrane protein fragment, binds phosphatidylserine from bilayer mixtures with phosphatidylcholine: a (2)H-NMR study.
Biophys J. 2000 Nov;79(5):2624-31
Authors: Roux M, Beswick V, Coïc YM, Huynh-Dinh T, Sanson A, Neumann JM
PMP1 is a 38-residue plasma membrane protein of the yeast Saccharomyces cerevisiae that regulates the activity of the H(+)-ATPase. The...
nmrlearner
Journal club
0
11-19-2010 08:29 PM
[NMR paper] The structure of the O-linked carbohydrate chain of bovine seminal plasma protein PDC
The structure of the O-linked carbohydrate chain of bovine seminal plasma protein PDC-109 revised by H-NMR spectroscopy A correction.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The structure of the O-linked carbohydrate chain of bovine seminal plasma protein PDC-109 revised by H-NMR spectroscopy A correction.
FEBS Lett. 1996 May 27;387(1):99-100
Authors: Gerwig GL, Calvete JJ, Töpfer-Petersen E, Vliegenthart JF
nmrlearner
Journal club
0
08-22-2010 02:27 PM
[NMR paper] Transduction of reducing power across the plasma membrane by reduced glutathione. A 1
Transduction of reducing power across the plasma membrane by reduced glutathione. A 1H-NMR spin-echo study of intact human erythrocytes.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Transduction of reducing power across the plasma membrane by reduced glutathione. A 1H-NMR spin-echo study of intact human erythrocytes.
Eur J Biochem. 1993 Aug 1;215(3):711-8
Authors: Ciriolo MR, Paci M, Sette M, De Martino A, Bozzi A, Rotilio G
...
nmrlearner
Journal club
0
08-22-2010 03:01 AM
[NMR paper] 1H NMR-based determination of the three-dimensional structure of the human plasma fib
1H NMR-based determination of the three-dimensional structure of the human plasma fibronectin fragment containing inter-chain disulfide bonds.
Related Articles 1H NMR-based determination of the three-dimensional structure of the human plasma fibronectin fragment containing inter-chain disulfide bonds.
J Biol Chem. 1993 Apr 25;268(12):8580-9
Authors: Kar L, Lai CS, Wolff CE, Nettesheim D, Sherman S, Johnson ME
Human plasma fibronectin is a plasma glycoprotein that plays an important role in many biological processes. It consists of two...
nmrlearner
Journal club
0
08-21-2010 11:53 PM
[NMR paper] 1H NMR spectroscopic studies on the interactions between human plasma antithrombin II
1H NMR spectroscopic studies on the interactions between human plasma antithrombin III and defined low molecular weight heparin fragments.
Related Articles 1H NMR spectroscopic studies on the interactions between human plasma antithrombin III and defined low molecular weight heparin fragments.
Biochemistry. 1992 Mar 3;31(8):2286-94
Authors: Horne A, Gettins P
The effects of length and composition upon the antithrombin-binding properties of heparin have been investigated for two series of structurally related heparin oligosaccharides. Each...
nmrlearner
Journal club
0
08-21-2010 11:41 PM
[NMR paper] 1H NMR studies of reactions of copper complexes with human blood plasma and urine.
1H NMR studies of reactions of copper complexes with human blood plasma and urine.
Related Articles 1H NMR studies of reactions of copper complexes with human blood plasma and urine.
Biochem Pharmacol. 1992 Jan 22;43(2):137-45
Authors: Bligh SW, Boyle HA, McEwen AB, Sadler PJ, Woodham RH
Reactions of the copper complexes Cu(II)Cl2, 2-, and + (where DIPS is 3,5-diisopropylsalicylate and DMP is 2,9-dimethylphenanthroline) with human blood plasma and urine have been studied by 500 MHz 1H NMR spectroscopy, and CD spectroscopy has been used to...
nmrlearner
Journal club
0
08-21-2010 11:41 PM
[NMR paper] Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.
Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.
Related Articles Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.
Int J Pept Protein Res. 1991 Sep;38(3):260-6
Authors: Era S, Kuwata K, Sogami M, Kato K, Watari H
Bovine plasma albumin (BPA) has 17 disulfide bonds and approximately one SH group at Cys-34 which catalyzes the intramolecular SH, S-S exchange reaction in the alkaline region at low ionic strength, resulting in the formation of the aged form (A-form). 1) Fractions of...
nmrlearner
Journal club
0
08-21-2010 11:12 PM
[NMR paper] Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.
Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.
Related Articles Circular dichroic and 1H-NMR studies on the aged form of bovine plasma albumin.
Int J Pept Protein Res. 1991 Sep;38(3):260-6
Authors: Era S, Kuwata K, Sogami M, Kato K, Watari H
Bovine plasma albumin (BPA) has 17 disulfide bonds and approximately one SH group at Cys-34 which catalyzes the intramolecular SH, S-S exchange reaction in the alkaline region at low ionic strength, resulting in the formation of the aged form (A-form). 1) Fractions of...
NMR in plasma (6 replies)
Linear Mode
