Question from NMRWiki Q&A forum negative NMR peaks

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] negative NMR peaks

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-21-2011, 03:40 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

negative NMR peaks

negative NMR peaks

I am very new to NMR and have little experience of it. However I am trying to use it to determine the composition of a mixture. A spectra which I have just obtained contains negative peaks which are giving me incorrect inegrals.

What would cause this? and is there any way to correct it? I can't find much in literature regarding this.

Thanks for your help

Dean

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMRpipe Yahoo group] Asymmetric peaks Asymmetric peaks Dear users, I have a NOESY filtered in F2 but not in F1 so I expect symmetric peaks (not usefuls) and asymmetryc peaks. Does NMRPipe contains a script/command More...	NMRpipe Yahoo group news	News from other NMR forums	0	07-06-2011 06:21 PM
[NMR paper] Negative entropy of halothane binding to protein: 19F-NMR with a novel cell. Negative entropy of halothane binding to protein: 19F-NMR with a novel cell. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Negative entropy of halothane binding to protein: 19F-NMR with a novel cell. Biochim Biophys Acta. 1997 Mar 15;1334(2-3):117-22 Authors: Yoshida T, Tanaka M, Mori Y, Ueda I An obvious difficulty of the study of binding of volatile anesthetics to proteins is to prevent loss of the ligand during the procedure. A novel NMR tube was designed that...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Negative entropy of halothane binding to protein: 19F-NMR with a novel cell. Negative entropy of halothane binding to protein: 19F-NMR with a novel cell. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Negative entropy of halothane binding to protein: 19F-NMR with a novel cell. Biochim Biophys Acta. 1997 Mar 15;1334(2-3):117-22 Authors: Yoshida T, Tanaka M, Mori Y, Ueda I An obvious difficulty of the study of binding of volatile anesthetics to proteins is to prevent loss of the ligand during the procedure. A novel NMR tube was designed that...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits the DNA-binding activity of the positive-acting nitrogen regulatory protein NIT2. Related Articles The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits the DNA-binding activity of the positive-acting nitrogen regulatory protein NIT2. Biochemistry. 1995 Jul 11;34(27):8861-8 Authors: Xiao X, Fu YH, Marzluf GA Structural genes of the nitrogen regulatory circuit of the filamentous fungus Neurospora crassa are under the control of...	nmrlearner	Journal club	0	08-22-2010 03:50 AM
[NMR paper] Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species. Related Articles Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species. Biochem Genet. 1991 Oct;29(9-10):447-59 Authors: Young JL, Marzluf GA In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species. Related Articles Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species. Biochem Genet. 1991 Oct;29(9-10):447-59 Authors: Young JL, Marzluf GA In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nit Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nitrogen circuit of Neurospora crassa. Related Articles Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nitrogen circuit of Neurospora crassa. Mol Gen Genet. 1990 Jun;222(1):120-8 Authors: Young JL, Jarai G, Fu YH, Marzluf GA In Neurospora the expression of a set of unlinked structural genes, which allows utilization of various nitrogen-containing compounds, is controlled by the positive-acting nit-2 gene and the negative-acting...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off