Question from NMRWiki Q&A forum N15 NNH separated NOESY

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] N15 NNH separated NOESY

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-21-2012, 03:43 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

N15 NNH separated NOESY

N15 NNH separated NOESY

Dear friends,

I came across one reference which describe N15 NNH separated NOESY experiment but I am not able to get more information about this experiment from other sources. Is this experiment useful for providing restraints for protein structure calculation. what kind of information this experiment can provide and how to set up. If anyone has references to suggest, please suggest.

Thanks & Regards

Arun

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum Auto peak picking of N15Edit NOESY and C13 Edit NOESY spectrum Dear friends, I am in process of doing structure calculation of a dimeric protein. The problem I faced is regarding the assignment of N15Edit NOESY and C13 Edit NOESY spectrum.I have already processed the fid data and converted to sparky ucsf format. Is there options available in sparky for auto peak picking, integration and assignment of my NOESY spectrum? And second query is regarding the generation of input files for structure calculation. In our Lab we are using ARIA 2.1 software for structure calculation? Is there any...	nmrlearner	News from other NMR forums	0	12-14-2011 07:14 PM
[Question from NMRWiki Q&A forum] NOESY+WET 2D artifacts NOESY+WET 2D artifacts When i run 2d noesy with wet suppression (Hypercomplex with phase=1,2, nt=32, so phasecycle errors should be excluded)), i always found strong artifact ("mirrored diagonal") in the center of thespectrum. I know it is known artifact in Hypercomplex method, but never observed it without wet. How can I avoid this artifact? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	09-07-2011 08:21 PM
[Question from NMRWiki Q&A forum] Beginner: Help understanding 3D NOESY Beginner: Help understanding 3D NOESY Hi, I am a person with a Computer Science background who is dealing with some NMR dataset. I am concerned with the assignment of NMR peaks from several experiments. For structure determination, I am dealing with 3D-NOESY data. I am finding it a little hard to comprehend what the several peaks mean here. I understand what the peaks in a 2D dataset like 1H-1H NOESY dataset mean but not on higher dimensions. I do not understand the nomenclature followed in the naming of NOESY experiments because I feel a little lost in all the technical details in the...	nmrlearner	News from other NMR forums	0	06-10-2011 01:41 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Thank you for your replies. The processing script of TOCSY (NH plane): $NMRTXT/ext.xz.com fid/test%03d.fid xz001.fid nmrPipe -in xz001.fid \ ... -out xzf.ft2 More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-29-2011 11:00 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Hi Iris, Another question is what do you mean by the NH vs HH planes in regards to the 3D matrix (a single 3D peak can't be positive viewed along one axis and More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-27-2011 10:40 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY Re: N15 separated NOESY and TOCSY Hi Iris, It's difficult to diagnose the problem without seeing the processing script, but what phase correction was used in the 15N dimension? If the initial More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-26-2011 10:34 PM
[NMRpipe Yahoo group] N15 separated NOESY and TOCSY N15 separated NOESY and TOCSY Hello, I did a N15 separated TOCSY experiment and used the following script: #!/bin/csh bruk2pipe -in ./ser \ -bad 0.0 -aswap -DMX -decim 2632 -dspfvs 20 More...	NMRpipe Yahoo group news	News from other NMR forums	0	05-26-2011 10:34 PM
1H-13C Separated Local Field Spectroscopy of Uniformly 13C Labeled Peptides and Prote 1H-13C Separated Local Field Spectroscopy of Uniformly 13C Labeled Peptides and Proteins Publication year: 2010 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 1 July 2010</br> Eugene C., Lin , Chin H., Wu , Yuan, Yang , Christopher V., Grant , Stanley J., Opella</br> By incorporating homonuclear decoupling on both the 1H and 13C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100% labeled with 13C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated...	nmrlearner	Journal club	0	08-16-2010 03:50 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off