Question from NMRWiki Q&A forum Low resolution of pepitde samples

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[Question from NMRWiki Q&A forum] Low resolution of pepitde samples

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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06-07-2011, 01:01 AM

nmrlearner

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Low resolution of pepitde samples

Low resolution of pepitde samples

Dear friends,

I am now trying to record the NOESY spectrum of my 30 amino acids peptide on DRX600 with a TXI cryo probe in a tris buffer with 10% D2O. But I got a quite bad resolution of my data compared with reported one. I used good tubes and reagents, tuned and matched well. For shimming I used 1D1H gradshim. Do I need to perform even better shimming? And how can I do it? Or maybe it is because of something else?

Could you offer me some suggestions?

Thank you very much in advance!

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