In a normal fully decoupled carbon experiment, can you integrate equivalent carbons? In other words , do all CH3's get the same signal enhancement so that you could integrate CH3's comparatively?
I imaginable that the issue is still that T1 relaxation would make this still impossible.
How about with Cr(acac)3 added? Could you then integrate equivalent carbons?
I understand that you can set up an experiment specifically for integrating carbon spectra, but I'm trying to see if you can get any integration data from a typical decoupled carbon NMR.
Check if somebody has answered this question on NMRWiki QA forum
Did you find this post helpful? |
Similar Threads
Thread
Thread Starter
Forum
Replies
Last Post
[NMR paper] NMR structure of Mistic, a membrane-integrating protein for membrane protein expressi
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.
Related Articles NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.
Science. 2005 Feb 25;307(5713):1317-21
Authors: Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an...
nmrlearner
Journal club
0
11-24-2010 11:14 PM
[NMR analysis blog] On integrating overlapped peaks
On integrating overlapped peaks
Following up from the integration problem raised in my previous post and before I delve into Line Fitting, I would like to give you a quick update on some progress we have recently done in Mnova to facilitate the accurate integration of peaks in those cases in which a multiplet is contaminated by one or several extraneous peaks (e.g. a solvent peak).
Consider the following spectrum predicted using NMRPredict Desktop
http://3.bp.blogspot.com/_-MfflvAgRls/S08KOThgdBI/AAAAAAAAAkU/RsKSptjbJ1g/s400/Integration1.jpg
As expected (this is a perfect synthetic...
Integrating 13C spectra
Linear Mode
