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[Question from NMRWiki Q&A forum] HSQC perturbation
HSQC perturbation
Dear All,
I am trying to determine the interaction between 2 proteins. Is there any reference or literature which report the determination of binding constant between 2 proteins in which intensity drop of HSQC cross peaks can be used as the measuring parameter. As when I titrate these 2 proteins only change which I observe is intensity drop. I tried other biophysical techniques to determine binding constant, but results are not satisfactory.
Any suggestion would be useful
Regards
nmrlearner
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09-24-2013 02:18 AM
[Question from NMRWiki Q&A forum] what went wrong?
what went wrong?
a query again?
I recorded an DQF on deuterated solid sample. The basic pulse programme taken from bruker, optimised the pulse and kept the proper short delay between the last two 90 pulses? phase cycling is same as mentioned in bruker? I got empty spectrum with very poor diagonal and no cross peaks?
what could possibly go wrong ? does these delays depend on higher magnetic field strengths? or pulse imperfections cause collapsed spectrum or empty spectrum?
the spectra i recorded on 700 MHz with 14 KHz MAS.
nmrlearner
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11-18-2011 06:51 PM
[Question from NMRWiki Q&A forum] What's wrong with this spectrum? Bruker TPPI 2D TOCSY
What's wrong with this spectrum? Bruker TPPI 2D TOCSY
Hi,
I think this is probably a basic problem but I'm a bit inexperienced with NMR. I'm just trying to process an ethanol TOCSY spectrum from the biomolecular magnetic resonance data bank, but strange peaks are appearing at the very edge of the data.
The spectrum should look like this (processed in SpinWorks):
<img src="http://qa.nmrwiki.org/upfiles/12889441489353594.png" width="70%"</img>
nmrlearner
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11-05-2010 12:20 PM
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl
Best pulse sequence for HSQC in Guanidine-HCl
Hello,
I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63.
Using WGgNhsqc, the protein...
nmrlearner
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10-23-2010 07:42 AM
[Question from NMRWiki Q&A forum] data processing by nmrPipe
data processing by nmrPipe
Hi all,I am very new to nmrPipe. I want to achieve the following steps. It would be very kind if somebody helps.(1) I have a 2D bruker data (t2= 4k points, t1= 32 points) and I want to do Fourier transform in the F2 dimension,phase etc. (2) and then extract the 32 spectra as 1Ds and also convert these 32 spectra in ascii format.(3) fit the peaks with a gaussian or Lorentzian function.(4) integrate the peak and save the integrals and time points as an ascii file.I only managed till the 1st step.Please help..!!
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nmrlearner
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10-05-2010 02:11 PM
[Question from NMRWiki Q&A forum] Examples of protein degradation eg HSQC?
Examples of protein degradation eg HSQC?
Anyone know any websites or papers that show protein degradation in an HSQC for example? I wanted to know what to look for in old samples when I have them. Either that, or could someone put one up on the wiki for me to look at?
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nmrlearner
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08-22-2010 02:30 AM
[U. of Ottawa NMR Facility Blog] Hsqc - tocsy
HSQC - TOCSY
There are many different NMR techniques used to probe molecular structure. Two very useful methods are the HSQC (or HMQC) sequence used to obtain heteronuclear coupling correlations and the TOCSY sequence used to look at extended homonuclear coupling correlations. These are among the most widely used pulse sequences by chemists to help elucidate the molecular structure of organic molecules. The sequences can also be combined into a single HSQC-TOCSY sequence which is simply an HSQC followed by a TOCSY spin lock. For the case of 1H and 13C in organic molecules, the HSQC-TOCSY...
nmrlearner
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08-21-2010 08:15 PM
[Stan NMR blog] Are Bruker and Jeol NMR data wrong?
Are Bruker and Jeol NMR data wrong?
Recent FID's acquired with Bruker and Jeol spectrometers exhibit an anomalous group delay artifact ...
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