Question from NMRWiki Q&A forum incorrect processing of 2D HSQC in NMRPipe - what is wrong?

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[Question from NMRWiki Q&A forum] incorrect processing of 2D HSQC in NMRPipe - what is wrong?

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04-09-2011, 12:17 AM

nmrlearner

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incorrect processing of 2D HSQC in NMRPipe - what is wrong?

incorrect processing of 2D HSQC in NMRPipe - what is wrong?

i'm trying to process an HSQC by using standard scripts of NMRpipe, and get this:

what is wrong in my processing? at the spectrometer the spectrum looked nice, so it's a matter of processing...

conversion:

bruk2pipe -in ../Bruker/ser -bad 0.0 -aswap -DMX -decim 2080 -dspfvs 20 -grpdly 67.9842 \ -xN 4096 -yN 512 \ -xT 2048 -yT 256 \ -xMODE DQD -yMODE States-TPPI \ -xSW 9615.38 -ySW 2100 \ -xOBS 599.762823 -yOBS 60.780 \ -xCAR 4.707 -yCAR 115.00 \ -xLAB HN -yLAB N \ -ndim 2 -aq2D States-TPPI \ -out ../Data/test.fid -verb -ovprocessing:

nmrPipe -in ./test.fid \| nmrPipe -fn POLY -time \| nmrPipe -fn SINE -c 0.5 -off 0.45 -end 0.98 -pow 2 \| nmrPipe -fn ZF -auto \| nmrPipe -fn FT \| nmrPipe -fn PS -p0 -20.0 -p1 0.0 -di \| nmrPipe -fn EXT -left -sw \| nmrPipe -fn TP \| nmrPipe -fn SINE -c 0.5 -off 0.45 -end 0.98 -pow 2 \| nmrPipe -fn ZF -auto \| nmrPipe -fn FT \| nmrPipe -fn PS -p0 0.0 -p1 0.0 -di \| nmrPipe -fn TP \| nmrPipe -out ./test.ft -ovthanks for the tips!

Nadia

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