I was considering the possibility of trying some fluorine NMR and was wanting to know if anyone knew any simple ways of incorporating Fluorine probes into my protein relatively cheaply or using E.coli. I would be particularly interested in using fluro-tryptophan for dual use as NMR and florescence probes. Also does anyone have a feel for the size limitations of 19F probes literature always quotes around 100KDa but is it possible to go considerably higher, say 500?
any advice would be great
Tom
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incorporating fluorine into proteins
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