IS THEIR ANY NMR EXPERMENT TO KNOW THE IMPACT OF DEUTERATION ON RELAXATION REATES OF C13,N15,H ALFA , H BETA OF DEURATED PROTEIN (RANDOMLY DEUTERTED) Regards
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Water proton spin saturation affects measured protein backboneN spin relaxation rates
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Publication year: 2011
Source: Journal of Magnetic Resonance, Available online 1 October 2011</br>
Kang*Chen, Nico*Tjandra</br>
Protein backboneN NMR spin relaxation rates are useful in characterizing the protein dynamics and structures. To observe the protein nuclear-spin resonances a pulse sequence has to include a water suppression scheme. There are two commonly employed methods, saturating or dephasing the water spins with pulse field gradients and keeping them unperturbed with flip-back pulses....
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http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja201020c/aop/images/medium/ja-2011-01020c_0002.gif
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