How would you compare solving NMR structure of a smaller peptide vs that of globular protein?
 

How would you compare solving NMR structure of a smaller peptide vs that of globular protein?

By smaller peptide I mean somewhere around 15-20 aminoacids, having a cycle or two that constrain the geometry.

Also - I am interested in getting as much detail about the structure of the peptide as possible.

Do you typically need to record NOE buildup curves in such cases?

Given that side-chains of the peptide are unlikely to be packed like in the core of the globular protein - do you include side-chain NOE's into structure calculations?

Could you recommend any citations?

Thank you!.



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