Hi all, Although it sounds silly, but the arrows (you can see as elongated triangle) connecting peaks and their labels in Sparky sometimes mess up the whole
Peak height versus peak volume
Given a standard NOESY-based protein structure determination: Does anyone have any information on the benefits of measuring peak intensity by a volume integration method rather than simply measuring the peak height.
Obviously integration is theoretically more accurate, but does it make any difference to the quality of the structures produced? especially if peak lineshapes are comparable?
I was hoping to find some study comparing structures produced by both methods.....
I'm also curious about the benefits of distance-calbrating NOEs to a curve rather than simply putting restraints...
paul
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09-15-2015 07:48 PM
[CNS Yahoo group] MTZ label
MTZ label
Hi I am facing the problem of putting correct label in the MTZ file. Ctruncate (ccp4 GUI) programme uses some default label which is not sufficient to run
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nmrlearner
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04-15-2012 12:33 AM
[NMRpipe Yahoo group] Re: How to remove the wiggles in 2D 13C-13C spectrum?
Re: How to remove the wiggles in 2D 13C-13C spectrum?
Hi Hoa, Check out this older post from Frank on the Yahoo NMRPipe user group forum: http://tech.groups.yahoo.com/group/nmrpipe/message/754 Basically, a first
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01-11-2011 01:02 AM
[NMRpipe Yahoo group] Re: How to remove the wiggles in 2D 13C-13C spectrum?
Re: How to remove the wiggles in 2D 13C-13C spectrum?
Hello Ryan McKay and nmrPipe users, Yes, I replaced the first 3 points as you suggested in your last email with both LP and LPC. Unfortunately, the wiggles
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01-10-2011 12:59 AM
[NMRpipe Yahoo group] Re: How to remove the wiggles in 2D 13C-13C spectrum?
Re: How to remove the wiggles in 2D 13C-13C spectrum?
Did you try replacing the first 3 points with back prediction? The first points are the most likely culprits. Then solvent refocusing later in the experiment
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01-08-2011 12:17 AM
[NMRpipe Yahoo group] Re: How to remove the wiggles in 2D 13C-13C spectrum?
Re: How to remove the wiggles in 2D 13C-13C spectrum?
So sorry for this late reply. Thank you very much to all of you for your comments, suggestions and corrections. Unfortunately, the simple sine and sine square
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01-08-2011 12:17 AM
[NMRpipe Yahoo group] Re: How to remove the wiggles in 2D 13C-13C spectrum?
Re: How to remove the wiggles in 2D 13C-13C spectrum?
Try replacing the first few points of the acquisition with back prediction ... Try replacing the first 2 or 3 points. Best wishes, Ryan On 2011-01-06, at 10:30
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01-07-2011 12:01 AM
[NMR paper] Automated peak picking and peak integration in macromolecular NMR spectra using AUTOP
Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
Related Articles Automated peak picking and peak integration in macromolecular NMR spectra using AUTOPSY.
J Magn Reson. 1998 Dec;135(2):288-97
Authors: Koradi R, Billeter M, Engeli M, Güntert P, Wüthrich K
A new approach for automated peak picking of multidimensional protein NMR spectra with strong overlap is introduced, which makes use of the program AUTOPSY (automated peak picking for NMR spectroscopy). The main elements of this program are a novel...