Question from NMRWiki Q&A forum How to obtain a good COSY spectrum of a paramagnetic compound

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] How to obtain a good COSY spectrum of a paramagnetic compound

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-22-2013, 04:43 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

How to obtain a good COSY spectrum of a paramagnetic compound

How to obtain a good COSY spectrum of a paramagnetic compound

How to set up the experiment in order to obtain a good COSY spectrum of Cu(II) compound?

I have tried to do it by just changing the spectrum width of both dimension, with an hour of acquistion time, but no cross peak can be observed between any signal.

Thanks

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
How to obtain a good COSY spectrum of a paramagnetic compound How to set up the experiment in order to obtain a good COSY spectrum of compound that containing Cu(II), which should hyperfine shift from 150ppm to -50 ppm? I have tried to do it by just changing the spectrum width of both dimension, with an hour of acquistion time, but no cross peak can be observed between any signal.	kcsham2	NMR Questions and Answers	0	05-22-2013 07:42 AM
A.5.1 Deduce the structure of a compound given information from 1H NMR spectrum IB Chemistry SL A.5.1 Deduce the structure of a compound given information from 1H NMR spectrum IB Chemistry SL http://i.ytimg.com/vi/LBHXbh3unBs/default.jpg A.5.1 Deduce the structure of a compound given information from 1H NMR spectrum IB Chemistry SL How an NMR machine works is not in the SL syllabus -- but you do need to know how to interpret a simplified spectrum. The number of peaks = the number of dif... From:Richard Thornley Views:8463 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif...	nmrlearner	NMR educational videos	0	05-08-2013 02:49 PM
Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification Two-dimensional concurrent HMQC-COSY as an approach for small molecule chemical shift assignment and compound identification Abstract Chemical shift assignment is the first step toward the structure elucidation of natural products and other chemical compounds. We propose here the use of 2D concurrent HMQC-COSY as an experiment for rapid chemical shift assignment of small molecules. This experiment provides well-dispersed 1Hâ??13C peak patterns that are distinctive for different functional groups plus 1Hâ??1H COSY connectivities that serve to identify adjacent groups. The COSY diagonal...	nmrlearner	Journal club	0	03-09-2011 04:19 AM
[NMR paper] Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: investigation of the active Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: investigation of the active site structure and the acid and alkaline transitions. Related Articles Paramagnetic 1H NMR spectrum of nickel(II) pseudoazurin: investigation of the active site structure and the acid and alkaline transitions. Inorg Chem. 2002 Dec 16;41(25):6662-72 Authors: Dennison C, Sato K The paramagnetic (1)H NMR spectrum of Ni(II) pseudoazurin possesses a number of resonances exhibiting sizable Fermi-contact shifts. These have been assigned to protons associated with...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] Simple multidimensional NMR experiments to obtain different types of one-bond dipolar Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. Related Articles Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. J Biomol NMR. 2001 Jan;19(1):63-7 Authors: de Alba E, Suzuki M, Tjandra N In order to measure residual dipolar couplings, the molecule under study has to be partially oriented in the presence of the magnetic field. It has been observed that some protein samples are not stable under the conditions imposed by...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
Nuclear Magnetic Resonance (NMR) spectrum of an organic compound http://farm4.static.flickr.com/3430/3920568119_34cc5627e6.jpg Nuclear Magnetic Resonance (NMR) spectrum of an organic compound More...	nmrlearner	NMR pictures	0	08-18-2010 01:12 PM
Script to obtain order parameters from a structure A Python script for prediction of order paramter from a structure is available from this website. The script is based on the following paper F. Zhang and R. Brüschweiler (2002) "Contact Model for the Prediction of NMR N-H Order Parameters in Globular Proteins" J. Am. Chem. Soc. 124(43), 12654-12655.	nmrlearner	NMR dynamics	0	05-07-2005 09:21 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off