Question from NMRWiki Q&A forum How important is calibrating a 90degree pulse for 2D?

		BioNMR > NMR community > News from other NMR forums
[Question from NMRWiki Q&A forum] How important is calibrating a 90degree pulse for 2D?

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-28-2012, 08:21 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

How important is calibrating a 90degree pulse for 2D?

How important is calibrating a 90degree pulse for 2D?

So it is pretty much always recommended to calibrate the 90 for 1H for any 2D experiment... Cosy, Noesy, HSQC, etc...

But when I have calibrated them, there is relatively little change... say from 7.32 to 7.65... Of course for all I know that could be a significant change...

Anyway, how 'off' does the 90 need to be to make a noticeable difference? If you have a concentrated sample, is it less sensitive to the 90?

Thanks!!

Check if somebody has answered this question on NMRWiki QA forum

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] How about calibrating a 13C 90 pulse? How about calibrating a 13C 90 pulse? Most 90 degree pulse calibration instructions I've run across seem to be for a 1H 90. What about a 13C 90 degree pulse? Anyone have an easy set of instructions for doing this? Is calibrating the 90 for 13C less important than the 1H 90 for 2D experiments? Thanks in advance! Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	06-22-2012 04:36 AM
[Question from NMRWiki Q&A forum] Calibrating 1H decoulpler pulse? Calibrating 1H decoulpler pulse? Recently while getting a loaner probe up and running, I noticed that the engineers simply had me set the decouple 90 to the same as the regular 90. I was under the impression that you wanted to use a lower power pulse for decoupling. Is setting the decoupling 90 to the same bascially a 'good enough' shortcut? If so, what's a better way to do it? and to determine it? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	06-22-2012 04:36 AM
SIMPSON - An important driver for numerical simulations in solid-state NMR spectroscopy. SIMPSON - An important driver for numerical simulations in solid-state NMR spectroscopy. SIMPSON - An important driver for numerical simulations in solid-state NMR spectroscopy. J Magn Reson. 2011 Sep 6; Authors: Bak M, Rasmussen JT, Nielsen NC Abstract We present a historical recollection on the development of the software package SIMPSON (SIMulation Package for SOlid-state Nmr). This covers a brief description of the underlying ideas and events leading to creation of SIMPSON and numerous auxiliary programs as well as comments on its...	nmrlearner	Journal club	0	09-10-2011 06:51 PM
NMR Provides a Quantitative Description of Protein Conformational Flexibility on Physiologically Important Timescales. NMR Provides a Quantitative Description of Protein Conformational Flexibility on Physiologically Important Timescales. NMR Provides a Quantitative Description of Protein Conformational Flexibility on Physiologically Important Timescales. Biochemistry. 2011 Mar 9; Authors: Salmon L, Bouvignies G, Markwick PR, Blackledge M A complete description of biomolecular activity requires an understanding of the nature and the role of protein conformational dynamics. In recent years novel NMR-based techniques have emerged that provide hitherto inaccessible...	nmrlearner	Journal club	0	03-11-2011 03:14 PM
[Question from NMRWiki Q&A forum] Correct mathematical formulas of the important window functions Correct mathematical formulas of the important window functions Hi, regarding my C++/Qt NMR processing application (see http://qa.nmrwiki.org/question/149/problem-reading-and-transforming-vendor-data-using-cqt or http://cutenmr.sourceforge.net/) I have a question concerning the window functions. I did research in the literature but could not find the "correct" mathematical formulas of the important window functions (exponential, gaussian, sine, sine squared, TRAF). For the gaussian function I found: exp(-(t*LB)^2)	nmrlearner	News from other NMR forums	0	09-19-2010 06:03 PM
[NMR paper] The carboxyl-terminal region of human interferon gamma is important for biological ac The carboxyl-terminal region of human interferon gamma is important for biological activity: mutagenic and NMR analysis. Related Articles The carboxyl-terminal region of human interferon gamma is important for biological activity: mutagenic and NMR analysis. Protein Eng. 1991 Feb;4(3):335-41 Authors: Lundell D, Lunn C, Dalgarno D, Fossetta J, Greenberg R, Reim R, Grace M, Narula S Deletion of nine amino acids from the carboxyl terminus of human IFN gamma (residues 138--146; LFRGRRASQ) resulted in a 7-fold increase in specific antiviral...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] Are the histidine residues of glutathione S-transferase important in catalysis? An as Are the histidine residues of glutathione S-transferase important in catalysis? An assessment by 13C NMR spectroscopy and site-specific mutagenesis. Related Articles Are the histidine residues of glutathione S-transferase important in catalysis? An assessment by 13C NMR spectroscopy and site-specific mutagenesis. J Biol Chem. 1991 Oct 15;266(29):19475-9 Authors: Zhang PH, Graminski GF, Armstrong RN To test the proposition that a histidine residue is essential in the catalytic mechanism of glutathione S-transferase, rat liver isoenzyme 3-3...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Are the histidine residues of glutathione S-transferase important in catalysis? An as Are the histidine residues of glutathione S-transferase important in catalysis? An assessment by 13C NMR spectroscopy and site-specific mutagenesis. Related Articles Are the histidine residues of glutathione S-transferase important in catalysis? An assessment by 13C NMR spectroscopy and site-specific mutagenesis. J Biol Chem. 1991 Oct 15;266(29):19475-9 Authors: Zhang PH, Graminski GF, Armstrong RN To test the proposition that a histidine residue is essential in the catalytic mechanism of glutathione S-transferase, rat liver isoenzyme 3-3...	nmrlearner	Journal club	0	08-21-2010 11:12 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off