Hello I am using satxfer1D experiment on varian unity plus 500 MHz spectrometer and vnmr (not vnmrJ!) software. I would like to determine dissociation constant from STD amplification factor (Io-Isat)/Io. So I need separate spectrum saturated at 30ppm to get off-resonance ligand integrals Io. Standard STD experiment give me only the difference between on- and off- resonance spectra (Io-Isat). Is the possibility to extract or to programm satxfer1D experiment to get what I want?
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[Question from NMRWiki Q&A forum] edasp window for 1H/19F double resonance experiment
edasp window for 1H/19F double resonance experiment
Hello to all, This time I am having problem with doing a double resonance experiment. The final goal is to do 1H/19F-HOESY, but I am confused in the edasp window:http://dl.dropbox.com/u/12535433/04.JPG
I remember I was doing 1H/13C heteronuclear shift correlation experiments before. I think for 13C it uses X-transmitter(!) and x100W amplifier. A normal 1D-19F works fine (H50W-X-XBB).It is a NMR300 and a QNP probe.Thanks is advance,Amin
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HNCB Triple Resonance Experiment (Varian)
Hello,
Has anyone come across this experiment? I see it listed but have not been able to find the pulse sequence. I have noticed that in some cases that the reference simply implies the HNCACB experiment running in such a way to allow complete transfer to the CB.
So is there an HNCB or is the only HNCB in the form of the HNCACB with the delay set to allow complete transfer to the CB?
Thanks in advance!!!
How to get off resonance ligand integral in STD experiment
Linear Mode
