Hello, I need to help solids lab at our UCI site - they have a custom probe and need to orient it properly in order to ease the shimming procedure.
My question is - how to determine orientation of X and Y shims?
We have 800 MHz 63 mm Oxford magnet (+ Varian UnityInova console)
Check if somebody has answered this question on NMRWiki QA forum
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[Stan NMR blog] Field shims headaches
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Answering a question about magnetic field shim systems on NMR spectrometers ...
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Source: Stan blog library
nmrlearner
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09-19-2010 05:43 AM
[Question from NMRWiki Q&A forum] How does removal and re-installation of probe affect shims?
How does removal and re-installation of probe affect shims?
If you remove and later (not too much later) reinstall the probe and you are confident that probe position inside the magnet does not change at all - would you expect the shim set to change?
Do eddy currents due to probe movement affect field homogeneity?
Thanks.
No need to purify protein to determine its structure by NMR
...if, of course, (1) your protein allows to be significantly overexpressed (2) adding inhibitors can protect your protein from proteolysis long enough to collect NMR spectra (3) your protein does not interact with inhibitors or molecules that have significant presence in crude cell-extract...
;)
Automated protein NMR structure determination in crude cell-extract.
Etezady-Esfarjani T, Herrmann T, Horst R, Wuthrich K.
J Biomol NMR. 2006 Jan;34(1):3-11.